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Anti-Desmin antibody produced in rabbit

Name: Anti-Desmin antibody produced in rabbit
Brand: SIGMA

Enhanced Validation

whole antiserum

Synonym(s):

Anti-Desmin, Desmin Detection Antibody, Rabbit Anti-Desmin

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00162258

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, WB

Citations:

Pricing and availability is not currently available.

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

chicken, bovine, human, mouse

enhanced validation

independent
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technique(s)

indirect immunofluorescence: 1:20 using cultured cells, indirect immunofluorescence: 1:20 using human skeletal muscle sections, western blot: 1:100 using animal or human skeletal muscle.

UniProt accession no.

P17661

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DES(1674)
mouse ... Des(13346)

Description

General description

Desmin is an intermediate filament protein found mainly in skeletal, cardiac, and smooth muscle cells. It acts as a marker for tumor origin. It also facilitates mechanical support for muscles, mitochondrial localization, myofibrillogenesis, gene expression regulation, and intracellular signalling. Anti-desmin antibody can be used for immunocytochemical localization of intermediate filaments in all types of muscle cells. It can also be used to stain a wide desmin band of 50,000 to 55,000 molecular weight using immunoblotting assays. Rabbit anti-desmin antibody reacts specifically with desmin in bovine, mouse, chicken and human.

Desmin is mapped to human chromosome 2q35. The desmin protein comprises of a head domain, four α-helical segments, three non-helical rod segments and a tail domain. It exists as dimer and tetramer assemblies. In smooth and striated muscles, desmin is localized in the periphery of Z-disk.

Immunogen

desmin from chicken gizzard

Application

Anti-Desmin antibody produced in rabbit has been used in:

immunohistochemistry of zebrafish intestines sections
immunodetection in fish embryo samples
immunolabeling of myofibers

Anti-desmin antibody can be used in immunocytochemistry and western immunoblotting. It can also be used in indirect immunofluorescence.

Biochem/physiol Actions

Desmin plays a crucial role in providing mechanical strength to muscle tissues. Mutations in desmin gene contributes to imbalance in skeletal proteins, resulting in desminopathies. Missense mutations in desmin gene leads to hypertrophic cardiomyopathy and systemic myopathy. Mutations in desmin gene impairs the stability of intermediary filaments in cardiomyocytes, contributing to pathophysiology of cardiac diseases.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	D1033	SAB4200707	M7523
Sigma-Aldrich D8281 Anti-Desmin antibody produced in rabbit	Sigma-Aldrich D1033 Monoclonal Anti-Desmin antibody produced in mouse	Sigma-Aldrich SAB4200707 Anti-Desmin antibody, Mouse monoclonal	Sigma-Aldrich M7523 Anti-Myosin (Skeletal) antibody produced in rabbit
description whole antiserum	description clone DE-U-10, ascites fluid	description clone DE-U-10, purified from hybridoma cell culture	description whole antiserum
biological source rabbit	biological source mouse	biological source -	biological source rabbit
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form whole antiserum	antibody form ascites fluid	antibody form purified from hybridoma cell culture	antibody form whole antiserum
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice
technique(s) indirect immunofluorescence: 1:20 using cultured cells, western blot: 1:100 using animal or human skeletal muscle., indirect immunofluorescence: 1:20 using human skeletal muscle sections	technique(s) indirect immunofluorescence: 1:40 using formalin-fixed, paraffin-embedded sections of human tissue, microarray: suitable, western blot: 1:200 using chicken gizzard extract/whole extract of mouse myoblast C2C12 cells	technique(s) immunoblotting: 0.5-1 μg/mL using whole extract of mouse myoblast C2C12 cells, immunofluorescence: 2-5 μg/mL using mouse myoblast C2C12 cells, immunohistochemistry: 2.5-5 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded Human Appendix, microarray: suitable	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human or animal skeletal muscle, indirect immunofluorescence: 1:20 using human or animal sletetal muscle

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Storage Class

10 - Combustible liquids

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Not applicable

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Key Documents

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Anti-Desmin antibody produced in rabbit

whole antiserum

Select a Size

About This Item

biological source

Quality Segment

conjugate

antibody form

antibody product type

clone

contains

species reactivity

enhanced validation

technique(s)

UniProt accession no.

shipped in

storage temp.

target post-translational modification

Gene Information

General description

Immunogen

Application

Biochem/physiol Actions

Disclaimer

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