This product has not been validated for immunohistochemistry (IHC). However, numerous citations exist using this product for IHC.
G1544
Anti-GFP, N-terminal antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-Green Fluorescent Protein
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| Size/SKU | Availability | Price |
|---|---|---|
100 μg | Available to ship TODAYfromMILWAUKEE | $571.00 |
About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IP, WB
Citations:
-
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates, western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Green fluorescent protein (GFP) chromophore contains hexapeptide fragment with Phe64-Ser-Tyr-Gly-Val-Gln69 aminoacid sequence. GFP structure contains a 11-stranded β-barrel and a central helix and corresponds to a molecular weight of 26 kDa.
Immunogen
synthetic peptide corresponding amino acids 3-17 of GFP.
Application
Anti-GFP, N-terminal antibody produced in rabbit has been used:
- in immunoblotting
- in the histochemistry and immunohistochemistry
- in the immunoprecipitation
Biochem/physiol Actions
Green fluorescent protein (GFP) has photochemical and diagnostic applications. It is also used widely in protein dynamics studies. The polyclonal anti-GFP, N-terminal rabbit antibody reacts specifically with GFP fusion proteins.
Specific for GFP fusion proteins.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
1 of 1
This Item | |||
|---|---|---|---|
| description ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution | description clone GSN149, purified from hybridoma cell culture | description from mouse IgG1κ (clones 7.1 and 13.1) | description affinity isolated antibody |
| antibody form affinity isolated antibody | antibody form purified from hybridoma cell culture | antibody form purified immunoglobulin | antibody form affinity isolated antibody |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level 100 |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate - |
| biological source rabbit | biological source mouse | biological source mouse | biological source rabbit |
| form buffered aqueous solution | form buffered aqueous solution | form lyophilized | form buffered aqueous glycerol solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
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Storage Class
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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