Key Documents

Anti-MAP1LC3A antibody

Name: Anti-MAP1LC3A antibody
Brand: SIGMA
Price: 161 USD
Availability: InStock

rabbit polyclonal

Synonym(s):

Anti-Lc3, LC3 Antibody - Anti-LC3 antibody produced in rabbit, Lc3 Antibody, Anti-MAP1 light chain 3-like protein 1, Anti-MAP1A/1B light chain 3A, Anti-MAP1A/1BLC3, Anti-MAP1A/MAP1B LC3A, Anti-MAP1ALC3, Anti-MAP1BLC3, Anti-MAP1LC3A, Anti-MAP1LC3B, Anti-Microtubule-associated protein 1 light chain 3 alpha, Anti-Microtubule-associated protein 1 light chain 3 beta, Anti-Microtubule-associated proteins 1A/1B light chain 3

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Size/SKU	Availability	Price
25 μL	Available to ship TODAYfromMILWAUKEE	$161.00
200 μL	Available to ship TODAYfromMILWAUKEE	$649.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IHC, IP, WB

Citations:

345

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Properties

Product Name

Anti-LC3 antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 16-18 kDa

species reactivity

mouse, human, rat

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): 5-10 μg using extracts of human U87 cells, western blot: 1-2 μg/mL using whole extracts of rat and mouse brain

UniProt accession no.

Q9H492

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAP1LC3A(84557), MAP1LC3B(81631)
mouse ... Map1lc3a(66734), Map1lc3b(67443)
rat ... Map1lc3a(362245), Map1lc3b(64862)

Description

General description

The gene LC3, also referred to as MAP1LC3A (microtubule associated protein 1 light chain 3 α), is a homologue of Apg8p necessary for autophagy in yeast. The cytosolic form upon processing gets localized to the autophagosome membranes. The gene is mapped to human chromosome 20q11.22. LC3 exists in cells as cytoplasmic LC3-I (18kDa) and LC3-II (16kDa).

Immunogen

Synthetic peptide corresponding to amino acids of human LC3A isoform a, conjugated to KLH via C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Application

Anti-LC3 antibody produced in rabbit has been used in:

western blotting
immunofluorescence
immunohistochemistry
filter retardation assay
immunocytochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (3 papers)
Immunohistochemistry (1 paper)
Western Blotting (21 paper)

Biochem/physiol Actions

The gene MAP1LC3A (microtubule associated protein 1 light chain 3 α) encodes a protein that serves as a marker of autophagosomes. It is found to be expressed on surviving oligodendrocytes in Nasu-Hakola disease brains and may be involved in the induction of oligodendrogliopathy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	L7543	SAB4200840	HPA007649
Sigma-Aldrich L8918 Anti-LC3 antibody produced in rabbit	Sigma-Aldrich L7543 Anti-LC3B antibody produced in rabbit	Sigma-Aldrich SAB4200840 Anti-Pro LC3C antibody, Mouse monoclonal	Sigma-Aldrich HPA007649 Anti-MAP1LC3A antibody produced in rabbit
description ~1 mg/mL, affinity isolated antibody, buffered aqueous solution	description ~1 mg/mL, affinity isolated antibody, buffered aqueous solution	description clone BTY-1, purified from hybridoma cell culture Affinity isolated antibody	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form -	antibody form affinity isolated antibody
Quality Level 200	Quality Level 200	Quality Level -	Quality Level 100
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source rabbit	biological source rabbit	biological source mouse	biological source rabbit
mol wt antigen 16-18 kDa	mol wt antigen 16-18 kDa	mol wt -	mol wt -
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in wet ice

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Storage Class

10 - Combustible liquids

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