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Anti-Nicastrin antibody produced in rabbit

Name: Anti-Nicastrin antibody produced in rabbit
Brand: SIGMA
Price: 643 USD
Availability: InStock

Enhanced Validation

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-ATAG1874

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Size/SKU	Availability	Price
0.2 mL	Available to ship TODAYfromMILWAUKEE	$643.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD03791914

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, WB

Citations:

$643.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 110 kDa

species reactivity

human, mouse, rat

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

microarray: suitable, western blot: 1:1,000 using minimum working antibody dilution using a whole cell extract of the HEK293 cell line stably transfected with human nicastrin.

UniProt accession no.

Q92542

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NCSTN(23385)

Description

General description

Nicastrin (NCSTN) is a type I transmembrane glycoprotein (709 amino acids) that interacts with both presenilin-1 (PS1) and presenilin-2 (PS2). This gene is located on human chromosome 1q23.

Immunogen

synthetic peptide corresponding to the C-terminus of human nicastrin (amino acids 693-709) conjugated to KLH. The corresponding sequence is identical in mouse.

Application

Anti-Nicastrin antibody produced in rabbit has been used in:

western blotting
immunoprecipitation
co-immunoprecipitation

Biochem/physiol Actions

Nicastrin has a key role in the regulation of presenilin-mediated cleavage of the b-amyloid precursor protein (b-APP) and Notch/GLP-1. Nicastrin binds to the membrane-tethered form of Notch and is essential for the intramembrane cleavage of Notch to generate Notch intracellular domain (NICD), which is involved in intracellular signaling. It has been suggested that nicastrin binds substrates of presenilin/g-secretase complexes or modulates g-secretase activity. Suppression of nicastrin expression in C. elegansembryos induces a subset of notch/glp-1 phenotypes similar to those induced by simultaneous null mutations in both presenilin homologues of C. elegans (sel-12 and hop-1). Thus, increasing evidence indicates that both nicastrin and presenilins are necessary components for the intramembrane proteolysis of proteins such as b-APP and Notch, and implicates a direct role for nicastrin in the pathogenesis of Alzheimer′s disease and in the regulation of Notch signaling in vivo.

Rabbit polyclonal anti-Nicastrin recognizes human nicastrin (110 kDa) by immunoblotting. Staining of nicastrin in immunoblotting is specifically inhibited with nicastrin immunizing peptide.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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description IgG fraction of antiserum, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution
Quality Level 200	Quality Level 100	Quality Level 200	Quality Level 200
antibody form IgG fraction of antiserum	antibody form IgG fraction of antiserum	antibody form IgG fraction of antiserum	antibody form IgG fraction of antiserum
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
technique(s) microarray: suitable, western blot: 1:1,000 using minimum working antibody dilution using a whole cell extract of the HEK293 cell line stably transfected with human nicastrin.	technique(s) immunofluorescence: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using methacarn-fixed, formic acid-treated sections of Alzheimer′s Disease (AD) brain, microarray: suitable, western blot: 1:10,000 using whole cell extract of PC12 rat adrenal pheochromocytoma cell line	technique(s) indirect immunofluorescence: 1:250-1:500 using mouse fibroblast NIH3T3 cells, western blot: 1:2,500-1:5,000 using whole cell extract of the rat pheochromocytoma PC12 cell line, western blot: 1:5,000-1:10,000 using whole cell extract of the A431 human epidermoid carcinoma cell line or a whole cell extract of the mouse fibroblast NIH3T3 cell line
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c