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P2317

10X PCR Buffer without MgCl2

Optimized for routine PCR without MgCl2

Synonym(s):

Magnesium-free PCR buffer

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About This Item

NACRES:
NA.52
UNSPSC Code:
41106306
Pricing and availability is not currently available.
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Quality Segment

form

liquid

feature

PCR Additives

technique(s)

PCR: suitable, whole genome amplification: suitable

color

colorless

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

General description

10X PCR Buffer II was tested at a final concentration of 1X (10mM Tris-HCl, pH 8.3 at 25 °C, 50mM KCl), in reactions containing 1-4mM MgCl2, each dNTP at 200 μM, primers defining an approximately 500 base pair region of λ DNA at 1.0μM each, λ DNA template at 1ng/100μL, and Taq DNA polymerase at 2.5 units/100μL. The reaction underwent 25 cycles of 94 °C to denature the double stranded DNA, 55 °C to anneal the DNA segments and 72 °C to extend the DNA segments. Following electrophoresis of the reaction products in 1.5% agarose gel, a single band of approximately 500 base pairs was visualized for PCRs containing 1-1.5mM MgCl2.

Application

10× PCR Buffer without MgCl2 has been used with Sigma′s PCR enzymes.
10X PCR Buffer without MgCl2 has been used as a component of polymerase chain reaction (PCR) amplification mixture for the mycotoxigenic mould DNA, parasite DNA and Fusarium oxysporum DNA amplification.It has also been used as a component of the PCR mix for the amplification of nuclear ribosomal internal transcribed spacer (ITS2) and the partial ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit rbcl gene for molecular and morphological characterization of Ulva sp from the Persian Gulf.

Features and Benefits

  • This product is tested for the absence of DNase and RNase.
  • Suitable for use with magnesium chloride.

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This Item
11699105001P219211699113001
10X PCR Buffer without MgCl2 Optimized for routine PCR without MgCl2

Sigma-Aldrich

P2317

10X PCR Buffer without MgCl2

PCR Buffer Without MgCl2, 10x concentrated solution, pkg of 3 × 1 mL

Roche

11699105001

PCR Buffer Without MgCl2, 10x concentrated

10X PCR Buffer Optimized for routine PCR with MgCl2 included

Sigma-Aldrich

P2192

10X PCR Buffer

MgCl2 Stock Solution pH 8.25-8.45 (25 °C), suitable for PCR, solution, pkg of 3 × 1 mL

Roche

11699113001

MgCl2 Stock Solution

description

Optimized for routine PCR without MgCl2

description

solution, pkg of 3 × 1 mL

description

Optimized for routine PCR with MgCl2 included

description

pH 8.25-8.45 (25 °C), suitable for PCR, solution, pkg of 3 × 1 mL

technique(s)

whole genome amplification: suitable, PCR: suitable

technique(s)

whole genome amplification: suitable, PCR: suitable

technique(s)

PCR: suitable, whole genome amplification: suitable

technique(s)

whole genome amplification: suitable, PCR: suitable

Quality Level

300

Quality Level

-

Quality Level

300

Quality Level

-

form

liquid

form

solution

form

liquid

form

solution

application(s)

agriculture

application(s)

-

application(s)

agriculture

application(s)

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

color

colorless

color

-

color

colorless

color

-

Storage Class

12 - Non Combustible Liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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