Yes, this product is suitable for coating culture surfaces such as plates, flasks, slides, and cover slips. After the coated surface is completely dry store in a sealed container or ziplock for up to one year. Do not use if coated surface becomes yellow or cloudy. Please see the link below to review the product datasheet for a similar item, P4707. This information may also be applied to P4832:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/244/731/p4707pis.pdf
P4832
Poly-ʟ-Lysine Hydrobromide
synthetic, mol wt 150,000-300,000, solution, 0.01%, suitable for cell culture
Poly-L-lysine solution
Synonym(s):
PLL Solution
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 50 mL | Available to ship TODAYfromMILWAUKEE | $140.00 |
About This Item
Biological source:
synthetic
Form:
solution
Technique(s):
cell culture | mammalian: suitable
Concentration:
0.01%
Mol wt:
150,000-300,000
Product Name
Poly-L-lysine solution, mol wt 150,000-300,000, 0.01%, sterile-filtered, BioReagent, suitable for cell culture
biological source
synthetic
Quality Level
sterility
sterile-filtered
product line
BioReagent
form
solution
mol wt
150,000-300,000
packaging
pkg of 50 mL
concentration
0.01%
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
solubility
water: soluble
shipped in
ambient
storage temp.
2-8°C
SMILES string
N([C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O)C(=O)[C@@H](N)CCCCN
InChI
1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
InChI key
WBSCNDJQPKSPII-KKUMJFAQSA-N
General description
L-lysine is a homopolypeptide having two enantiomeric forms, L and D lysine. Through its three reactive groups (the carboxylic acid and the α and ε amines), L-lysine has the capacity to generate various branched polymeric structures, including dendrimers, branched polylysine, and hyperbranched polylysine (PLL). Poly-L-lysine is a polymer made up of positively charged amino acid residues, with each lysine residue typically containing one HBr.
Application
Poly-L-lysine has been used:
- In coating 96-well plates for cell attachment in testing respiratory burst activity.
- In coating glass cover slips for immunofluorescent labeling of intracellular structures.[1]
- For pretreating 6-well plate in myosatellite cell isolation protocol.
- In coating coverslips for cell adhesion in E14 neuron culture to study possible mechanism of action of circRNA_01477 in neurons.
Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.
Biochem/physiol Actions
Poly‐l‐lysine (PLL) acts as polycationic polymers that have been extensively used to carry drugs, genes etc. across the cell membrane. Poly-L-lysine (PLL) has the capability to engage in the formation of polyelectrolyte complexes (PECs) by interacting with anionic entities such as polyanions, nucleic acid chains and some drugs.
Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.
Preparation Note
This product is supplied as a 0.01% sterile filtered solution in water, using a molecular weight of 150 - 300 kDa. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.
Other Notes
Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.
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This Item | |||
|---|---|---|---|
| biological source synthetic | biological source synthetic | biological source synthetic (organic) | biological source synthetic |
| sterility sterile-filtered | sterility sterile-filtered | sterility γ-irradiated | sterility - |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| concentration 0.01% | concentration 0.01% | concentration - | concentration 0.1 % (w/v) in H2O |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| mol wt 150,000-300,000 | mol wt 70—150 kDa | mol wt 70,000-150,000 | mol wt 150,000-300,000 |
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wgk
WGK 2
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.
Articles
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.
3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models
Molecular mechanisms underlying oxytocin-induced cardiomyocyte protection from simulated ischemia?reperfusion
Gonzalez-Reyes A, et al.
Molecular and Cellular Endocrinology, 412, 170-181 (2015)
Modulating the poly-L-lysine structure through the control of the protonation?deprotonation state of L-lysine
Stagi L, et al.
Scientific Reports (2022)
Effects of sex steroids on indices of protein turnover in rainbow trout (Oncorhynchus mykiss) white muscle
Cleveland BM and Weber GM
General and Comparative Endocrinology, 174(2), 132-142 (2011)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P4832-50ML | 04061834375265 |
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Can I coat a tissue culture plate and store it ? What is the best way to store for future use?
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