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P6282

Sigma-Aldrich

Poly-L-lysine hydrobromide

mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

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Synonym(s):
PLL HBr
CAS Number:
MDL number:
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.75

biological source

synthetic (organic)

Quality Level

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

mol wt

70,000-150,000

packaging

pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

4 μg/cm2

solubility

H2O: soluble 50 mL/vial, clear, colorless

shipped in

ambient

storage temp.

−20°C

SMILES string

Cl.NCCCCC(N)C(O)=O

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

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This Item
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Poly-L-lysine hydrobromide mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

P6282

Poly-L-lysine hydrobromide

Poly-L-lysine hydrobromide mol wt ≥300,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

P5899

Poly-L-lysine hydrobromide

Poly-L-lysine hydrobromide mol wt 4,000-15,000 by viscosity

P6516

Poly-L-lysine hydrobromide

Poly-L-lysine hydrobromide mol wt 15,000-30,000 by viscosity

P7890

Poly-L-lysine hydrobromide

biological source

synthetic (organic)

biological source

synthetic (organic)

biological source

-

biological source

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

Quality Level

200

Quality Level

200

Quality Level

-

Quality Level

-

form

lyophilized powder

form

lyophilized powder

form

solid

form

powder

mol wt

70,000-150,000

mol wt

≥300,000

mol wt

≤15,000 by MALLS, 4,000-15,000 by viscosity

mol wt

15,000-30,000 by viscosity

General description

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Application

Poly-L-lysine hydrobromide has been used to precoat glass bottom dishes used for in vitro cell culture.
Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.

Biochem/physiol Actions

Poly-L-lysine serves as a pre-coating substance to ensure better attachment of cells to culture surfaces. Research shows that plates coated with poly-L-lysine (PLL) demonstrated an accelerated cell growth rate. Culturing mesenchymal stem cells (MSCs) on PLL-coated substrates has been found to safeguard the stemness properties of these cells and inhibit senescence. PLL-coated plates have demonstrated enhanced MSC growth and up-regulated the expression of genes associated with cell adhesion, differentiation, proliferation, and signaling. Moreover, porous PLL-coated surfaces have shown improvements in cell proliferation and the osteogenic differentiation of MSCs.

Components

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Caution

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Analysis Note

Molecular weight based on viscosity. Also assayed by MALLS.

related product

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Articles

Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Protocols

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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