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Anti-Rab7 antibody produced in rabbit

Name: Anti-Rab7 antibody produced in rabbit
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Ras-Related Protein Rab7

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD08705509

Technique(s):

western blot: 2.5-5.0 μg/mL using extract of human A431 cells, indirect immunofluorescence: 5-10 μg/mL using rat NRK and mouse NIH-3T3 cells fixed and permeabilized with 3% paraformaldehyde followed by 0.4% saponin

Uniprot accession no.:

P51149

Application:

IF, WB

Citations:

Pricing and availability is not currently available.

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~23 kDa

species reactivity

mouse, human, rat

technique(s)

UniProt accession no.

P51149

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RAB7A(7879)
mouse ... Rab7(19349)
rat ... Rab7a(29448)

Description

General description

Rab7 is a member of the Rab family of small guanosine triphosphatases (GTPases). Rab proteins contain conserved regions involved in guanine nucleotide binding, and hypervariable COOH-terminal domains with a cysteine motif, implicated in subcellular targeting. Each Rab protein shows a characteristic subcellular distribution. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Ras-related protein Rab-7 (Rab7) is a member of the Rab family of small guanosine triphosphatases (GTPases).

Immunogen

Synthetic peptide corresponding to amino acid residues of human Rab7 with C-terminal added cysteine, conjugated to KLH.

Application

Anti-Rab7 antibody can be used in indirect immunofluorescence and immunoblotting.

Anti-Rab7 antibody produced in rabbit has been used in confocal microscopy.

Biochem/physiol Actions

Anti- Rab7 recognizes human, mouse and rat Rab7.

Rab family of small guanosine triphosphatases (GTPases) are central regulators of membrane trafficking between the different subcellular compartments of the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP-bound inactive and GTP-bound active states. Conversion from one state to the other is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment. Through their effector proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion. Rab7 is also involved in the maturation of late autophagic vacuoles. Among Rab7 effectors are RILP (Rab-interacting lysosome protein) that controls late endosomal and lysosomal transport by mediating the recruitment of dynein/dynactin motors, Rabring7 (Rab7- interacting ring finger protein),(10) and the hVPS34/p150 complex. Anti-Rab7 may be used as a marker for late endosomes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	R8779	ZRB1120	SAB5701027
Sigma-Aldrich R4779 Anti-Rab7 antibody produced in rabbit	Sigma-Aldrich R8779 Anti-Rab7 antibody, Mouse monoclonal	Sigma-Aldrich ZRB1120 Anti-Rab7A Antibody, clone 4E15 ZooMAb^® Rabbit Monoclonal	Sigma-Aldrich SAB5701027 Anti-RAB7 antibody produced in rabbit
description affinity isolated antibody, buffered aqueous solution	description ~2 mg/mL, clone Rab7-117, purified from hybridoma cell culture	description recombinant, expressed in HEK 293 cells	description -
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100
antibody form affinity isolated antibody	antibody form purified from hybridoma cell culture	antibody form purified antibody	antibody form -
biological source rabbit	biological source mouse	biological source rabbit	biological source rabbit
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
species reactivity mouse, human, rat	species reactivity bovine, human, monkey, mouse, chicken, rat	species reactivity mouse, human, monkey	species reactivity mouse, human, rat

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)