TS Answer: The protocol for this kit is published in the Certificate of Analysis. Please see the link below to review a sample document or access a lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/RAB0904#product-documentation
RAB0904
Rat Ins1 / Insulin ELISA Kit
Synonym(s):
Rat ELISA
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| Size/SKU | Availability | Price |
|---|---|---|
96 wells | Usually Ships in 4 Weeks. (Orders outside of US, please allow an additional 1-2 weeks for delivery) | $567.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
rat ... Ins1(24505)
Input:
sample type serum
sample type cell culture supernatant(s)
sample type plasma
sample type cell culture supernatant(s)
sample type plasma
Species reactivity:
rat
Storage temp.:
−20°C
Shipped in:
wet ice
$567.00
Usually Ships in 4 Weeks. (Orders outside of US, please allow an additional 1-2 weeks for delivery)
species reactivity
rat
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable
input
sample type serum
sample type cell culture supernatant(s)
sample type plasma
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 μIU/mL
standard curve range: 4.69–300 μIU/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
rat ... Ins1(24505)
General description
The antibody pair provided in this kit recognizes Rat Insulin.
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Rat Ins1 / Insulin ELISA Kit has been used to measure serum insulin concentration.
Biochem/physiol Actions
Certain organisms such as mice, rat and xenopus possess two non-allelic insulin genes. These genes are Ins1 and Ins2, which are both functional. They encode insulin that is produced in a 1:2 ratio, respectively in the pancreas. The two genes are differentially expressed in mouse. The Ins1 gene maps to mouse chromosome 19 in the telomeric region. The insulin that is synthesized in the pancreas is crucial in maintaining glucose homeostasis. Loss of Ins-1 and Ins-2 expression leads to insulin deficient mice with diabetes mellitus, ketoacidosis, liver steatosis and retarded growth.
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
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This Item | |||
|---|---|---|---|
| description - | description - | description This Rat/Mouse Insulin ELISA is used to measure & quantify Insulin levels in Metabolism & Endocrine research. | description for serum, plasma and cell culture supernatants |
| species reactivity rat | species reactivity porcine | species reactivity mouse, rat | species reactivity mouse |
| Gene Information rat ... Ins1(24505) | Gene Information Porcine ... INS(397415) | Gene Information - | Gene Information mouse ... Ins1(16333) |
| assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range standard curve range: 0.2-10 ng/mL, accuracy: 83-102%, sensitivity: 0.2 ng/mL, linearity: 79-133% | assay range inter-assay cv: <12% |
| input sample type serum | input sample type plasma | input sample type serum | input sample type plasma |
| technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable |
| detection method colorimetric | detection method colorimetric | detection method colorimetric (450nm/590nm) | detection method colorimetric |
Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Met. Corr. 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Storage Class
10 - Combustible liquids
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