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RDD002

HEPES

≥99.5%, crystalline powder, anhydrous, Redi-Dri

Synonym(s):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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1 kg
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$909.00
$681.75
2.5 kg
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$1,500.00

About This Item

Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12161700
EC Number:
230-907-9
MDL number:
Beilstein/REAXYS Number:
883043
eCl@ss:
32129211

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Product Name

HEPES, anhydrous, free-flowing, Redi-Dri, ≥99.5%

grade

anhydrous

Quality Segment

product line

Redi-Dri

assay

≥99.5%

form

crystalline powder

quality

free-flowing

pH

5.0-6.5 (25 °C, 238 g/L)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 500 mg/mL, clear, colorless

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:
  • In the novel vesicle-budding protocol as a component in stock solution and buffer solutions
  • As a component in intracellular solution for the preparation of brain-slice recording
  • As a component of wash buffer, elution buffer and desalting buffer during affinity protein purification
  • To maintain the pH at 7 in thermal denaturation assay of purified protein
  • In isocratic elution of protein using gel filtration
  • Along with NaCl in sample preparation for EPR (Electron Paramagnetic Resonance) spectroscopy

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

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description

anhydrous, free-flowing, Redi-Dri, ≥99.5%

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BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

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ULTROL® Grade, ≥99% dry basis (titration), zwitterionic N-substituted aminosulfonic acid buffer

description

OmniPur® Grade, >= 99%, Free Acid

grade

anhydrous

grade

-

grade

-

grade

-

assay

≥99.5%

assay

≥99.5% (titration)

assay

≥99% dry basis (titration)

assay

-

product line

Redi-Dri

product line

BioXtra

product line

-

product line

-

Quality Level

100

Quality Level

400

Quality Level

100

Quality Level

100

form

crystalline powder

form

powder

form

crystalline powder

form

fine powder

solubility

water: 500 mg/mL, clear, colorless

solubility

water: 500 mg/mL, clear, colorless

solubility

water: 1 M

solubility

-


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Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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