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SRE0095

Fast β-Glucuronidase, Recombinant

from limpets (Patella Vulgata), aqueous solution

Synonym(s):

β-Glucuronidase from limpets (Patella vulgata), β-D-Glucuronide glucuronosohydrolase

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Size/SKUAvailabilityPrice
10 mL

Available to ship TODAYfromMILWAUKEE

$476.00
25 mL

Available to ship TODAYfromMILWAUKEE

$1,060.00
50 mL

Available to ship TODAYfromMILWAUKEE

$2,150.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Recombinant:
expressed in proprietary host
Concentration:
300000-400000 units/mL

$476.00


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recombinant

expressed in proprietary host

Quality Segment

form

aqueous solution

concentration

300000-400000 units/mL

shipped in

ambient

storage temp.

2-8°C

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
More on the suitability of this product for hydrolysis of different drug-glucuronide substrates is available at this page.
The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes

Features and Benefits

Formulated with a higher enzyme concentration for applications requiring ultra-fast hydrolysis rates. Recombinantly expressed and purified to eliminate MAM esterase activity (6-Monoacetylmorphine→morphine). Representative analysis at high enzyme titer (50 un/μL β-glucuronidase) was less than 1% conversion of 6-MAM to morphine after 4 hours at 60 °C.

Physical form

Supplied as a solution in 100mM potassium acetate pH 5.2. It is free of detergents, carbohydrates or any other components that may interfere with sample preparation and analysis.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

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This Item
G8162G7646G8420
description

from limpets (Patella Vulgata), aqueous solution

description

aqueous glycerol solution, ≥5,000,000 units/g protein, pH 6.8 (biuret)

description

Type VII-A, lyophilized powder, 5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)

description

≥20,000 units/mg protein, recombinant, expressed in E. coli overproducing strain, lyophilized powder

concentration

300000-400000 units/mL

concentration

-

concentration

-

concentration

-

form

aqueous solution

form

aqueous glycerol solution

form

lyophilized powder

form

lyophilized powder

recombinant

expressed in proprietary host

recombinant

-

recombinant

-

recombinant

expressed in E. coli overproducing strain

shipped in

ambient

shipped in

wet ice

shipped in

-

shipped in

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

300


Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable



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