Key Documents

View All Documentation

Anti-β-Tubulin antibody, Mouse monoclonal

Name: Anti-β-Tubulin antibody, Mouse monoclonal
Brand: SIGMA
Price: 194 USD
Availability: InStock

clone TUB 2.1, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-β-Tubulin

Select a Size

Change View

Pack Size	Availability	Price
25 μL	Available to ship TODAYfromMILWAUKEE	$194.00
100 μL	Available to ship TODAYfromMILWAUKEE	$580.00
200 μL	Available to ship TODAYfromMILWAUKEE	$735.00

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

TUB 2.1, monoclonal

Application:

ARR, IEP, IF, IHC, WB

Citations:

278

$194.00

Available to ship TODAYDetails

A recombinant, preservative-free antibody is available for your target. Try ZRB1124

Request a Bulk Order

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

TUB 2.1, monoclonal

form

buffered aqueous solution

mol wt

antigen 55 kDa

species reactivity

human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

indirect immunofluorescence: 10-20 μg/mL using cultured chicken fibroblasts, microarray: suitable, immunoelectrophoresis: suitable, immunohistochemistry: suitable, western blot: 2-4 μg/mL using chicken fibroblasts cell extract

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Description

General description

β-Tubulin belongs to the subfamily of tubulin, which is the major building block of microtubules. β-Tubulin has a molecular weight of 55kDa. β-Tubulin structure is characterized with core of two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site, and a C-terminal domain that contains the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (βI (class I), βI (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V), and βVI (class VI)).

Monoclonal Anti-β-Tubulin (mouse IgG1 isotype) is derived from the hybridoma TUB 2.1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified rat brain tubulin.

Immunogen

tubulin from rat brain.

Application

Monoclonal Anti-β-Tubulin antibody produced in mouse has also been used in

2-dimensional electrophoresis
sperm motility
immunohistochemistry,
immunofluorescence[1]
immunoblotting[1]
immunocytochemistry[2]

Monoclonal Anti-β-Tubulin antibody produced in mouse has been used in western blotting.

Biochem/physiol Actions

Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to β-tubulin and inhibits NK cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. β-1 tubulin also known as class VI or TUBB1, plays a vital role in platelet production, and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).

The antibody recognizes all five isoforms of β-tubulin (β1-β5). It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections.

Physical form

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	T0198	T8328	T8578
Sigma-Aldrich T5201 Anti-β-Tubulin antibody, Mouse monoclonal	Sigma-Aldrich T0198 Anti-β-Tubulin antibody, Mouse monoclonal	Sigma-Aldrich T8328 Anti-β-Tubulin antibody, Mouse monoclonal	Sigma-Aldrich T8578 Anti-β-Tubulin III (neuronal) antibody, Mouse monoclonal
biological source mouse	biological source mouse	biological source mouse	biological source mouse
clone TUB 2.1, monoclonal	clone D66, monoclonal	clone AA2, monoclonal	clone 2G10, monoclonal
species reactivity human, rat, frog, moth, mouse, plant, rabbit, chicken, bovine, wheat, sea urchin, hamster	species reactivity human, bovine, chicken, rat, canine, Chlamydomonas reinhardtii, sea urchin (Lytechinus pictus and Paracentrotus lividus), mouse, hamster, Oxyrrhis marina (dinoflagellate), rabbit, monkey	species reactivity human, mouse, rat, bovine	species reactivity human, mouse, rat
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture
Gene Information human ... TUBB(203068), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB1(81027), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2A(7280), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383), TUBB2C(10383) mouse ... Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb1(104068), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2a(22151), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb2c(227613), Tubb3(22152) rat ... Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2(29212), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554), Tubb2c(296554)	Gene Information human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383) mouse ... Tubb1(545486), Tubb2a(22151), Tubb2c(227613) rat ... Tubb2(29212), Tubb2c(296554)	Gene Information human ... TUBB(203068)	Gene Information human ... TUBB3(10381) mouse ... Tubb3(22152) rat ... Tubb3(246118)

Still not finding the right product?

Explore all of our products under Anti-β-Tubulin antibody, Mouse monoclonal

— or —

Try our Product Selector Tool to narrow your options

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Involvement of argonaute proteins in gene silencing and activation by RNAs complementary to a non-coding transcript at the progesterone receptor promoter.

Chu Y

Nucleic Acids Research, 38(21), 7736-7748 (2010)

Cell cycle regulated phosphorylation of the telomere-associated protein TIN2.

Shuqun Yang et al.

PloS one, 8(8), e71697-e71697 (2013-08-27)

The protein TIN2 is a member of telomere-binding protein complex that serves to cap and protect mammalian chromosome ends. As a number of proteins in this complex are phosphorylated in a cell cycle-dependent manner, we investigated whether TIN2 is modified

Structure of the alpha beta tubulin dimer by electron crystallography.

Nogales E

Nature, 391(6663), 199-203 (1998)

View All Related Papers

Global Trade Item Number

SKU	GTIN
T5201-200UL	04061837347221
T5201-100UL	04061837347214

Questions

Reviews

No rating value