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Anti-γ-Tubulin Antibody

Name: Anti-γ-Tubulin Antibody
Brand: SIGMA
Price: 572 USD
Availability: InStock

mouse monoclonal, GTU-88

Synonym(s):

Anti-γ-Tubulin antibody, Mouse monoclonal

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Size/SKU	Availability	Price
100 μL	Check Cart for Availability	$572.00
0.2 mL	Check Cart for Availability	$760.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00677366

Conjugate:

unconjugated

Clone:

GTU-88, monoclonal

Application:

ELISA (i), ICC, WB

Citations:

1567

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Properties

Product Name

Anti-γ-Tubulin antibody, Mouse monoclonal, clone GTU-88, ascites fluid

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

GTU-88, monoclonal

mol wt

antigen 48 kDa

contains

15 mM sodium azide

species reactivity

rat, hamster, chicken, human, bovine, canine, Xenopus, mouse

technique(s)

immunocytochemistry: 1:5,000-1:10,000 using HeLa cells, indirect ELISA: suitable, western blot: 1:10,000 using cultured chicken fibroblast extract

isotype

IgG1

UniProt accession no.

P23258

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBG1(7283)
mouse ... Tubg1(103733)
rat ... Tubg1(252921)

Description

General description

γ-Tubulin (48kDa) is a ubiquitous and highly conserved protein within the microtubule organizing centers (MTOCs) in eukaryotic cells. γ-Tubulin is mapped to human chromosome 17q21.2 and codes for a member of the tubulin family. Human TUBG1 transcript is widely expressed in preimplantation embryos and brain.
Monoclonal Anti-γ-Tubulin (mouse IgG1 isotype) is derived from the GTU-88 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

synthetic γ-tubulin peptide, conjugated to KLH

Application

Monoclonal Anti-γ-Tubulin antibody has been used in western blotting, indirect immunofluorescence (IF) and immunofluorescence staining.
Monoclonal Anti-γ-Tubulin is also suitable for use in immunochemical applications such as immunoblotting, immunocytochemical staining of cultured cells and in ELISA.

Monoclonal Anti-gamma-Tubulin is suitable for use in immunochemical applications such as immunoblotting, immunocytochemical staining of cultured cells, and in ELISA.

Biochem/physiol Actions

γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. γ-Tubulin binds microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome. It functions as the microtubule nucleator at the MTOC. It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end. γ-Tubulin abundance is less than 1% of the level of either α- or β-tubulin. Overexpression of γ-tubulin is observed in lung cancer. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.

Monoclonal Anti-γ-Tubulin recognizes an epitope located in the N-terminal amino acids of γ-tubulin (48 kDa). Cross reactivity has been observed with human, bovine, dog, hamster, rat, mouse, chicken, and Xenopus γ-tubulin.

The antibody recognizes an epitope located within the N-terminal region of γ-tubulin.

Physical form

The product is provided as ascites fluid with 15 mM sodium azide as a preservative.

Preparation Note

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	T5326	T5192	T3559
Sigma-Aldrich T6557 Anti-γ-Tubulin antibody, Mouse monoclonal	Sigma-Aldrich T5326 Anti-γ-Tubulin antibody, Mouse monoclonal	Sigma-Aldrich T5192 Anti-γ-Tubulin antibody produced in rabbit	$Anti-γ-Tubulin antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich T3559 Anti-γ-Tubulin antibody produced in rabbit
description clone GTU-88, ascites fluid	description clone GTU-88, purified from hybridoma cell culture	description affinity isolated antibody, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution
clone GTU-88, monoclonal	clone GTU-88, monoclonal	clone polyclonal	clone polyclonal
biological source mouse	biological source mouse	biological source rabbit	biological source rabbit
conjugate unconjugated	conjugate -	conjugate unconjugated	conjugate unconjugated
antibody form ascites fluid	antibody form purified antibody (purified from hybridoma cell culture)	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum
species reactivity rat, hamster, chicken, human, bovine, canine, Xenopus, mouse	species reactivity rat, hamster, chicken, human, bovine, canine, Xenopus, mouse	species reactivity human, chicken	species reactivity human, chicken
Gene Information human ... TUBG1(7283) mouse ... Tubg1(103733) rat ... Tubg1(252921)	Gene Information human ... TUBG1(7283) mouse ... Tubg1(103733) rat ... Tubg1(252921)	Gene Information human ... TUBG1(7283), TUBG2(27175)	Gene Information human ... TUBG1(7283), TUBG2(27175)

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)