The recommended secondary antibody for western blot is Goat Anti-Mouse IgG Antibody, HRP conjugate, product number 12-349, available at https://www.sigmaaldrich.com/product/mm/12349
V9264
Anti-Vinculin Antibody
mouse monoclonal, hVIN-1
Sinonimo/i:
Monoclonal Anti-Vinculin antibody produced in mouse
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Cambia visualizzazione
| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
25 μL | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 149,00 € |
100 μL | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 541,00 € |
200 μL | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 697,00 € |
Informazioni su questo articolo
Conjugate:
unconjugated
Clone:
hVIN-1, monoclonal
Application:
ELISA (i), ICC, IHC, WB
Citations:
306
149,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Per il tuo target è disponibile un anticorpo ricombinante privo di conservanti. Prova ZMS1086
Servizio Tecnico
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Permettici di aiutartiNome del prodotto
Anti-vinculina monoclonale, clone hVIN-1, purified from hybridoma cell culture
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
hVIN-1, monoclonal
form
buffered aqueous solution
mol wt
antigen 116 kDa
species reactivity
frog, chicken, mouse, canine, human, bovine, rat, turkey
packaging
antibody small pack of 25 μL
concentration
~1.0 mg/mL
technique(s)
immunocytochemistry: 5-10 μg/mL using HS-68 human fibroblast cell culture, immunohistochemistry: suitable, indirect ELISA: suitable, western blot: 0.05-0.1 μg/mL using HS-68 total cell extract
isotype
IgG1
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)
General description
Anti-Vinculin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hVIN-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with vinculin purified from human uterus. Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletal muscle), neuromuscular junctions, and the membrane associated dense bodies of smooth muscle.
Immunogen
vinculina umana purificata da utero.
Application
Anti-Vinculin antibody, Mouse monoclonal has been used in
- immunoblotting
- immunofluorescence staining
- immunocytochemistry
- immunohistochemistry and enzyme linked immunosorbent assay (ELISA).
Biochem/physiol Actions
Marca specificamente la vinculina in corrispondenza dei contatti cellula-cellula e cellula-substrato. Reagisce fortemente con la vinculina umana. Mostra reattività crociata con la metavinculina dei muscoli lisci.
Vinculin functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Questo articolo | |||
|---|---|---|---|
| conjugate unconjugated | conjugate - | conjugate - | conjugate unconjugated |
| species reactivity frog, chicken, mouse, canine, human, bovine, rat, turkey | species reactivity human, monkey, rat, mouse, guinea pig, bovine, hamster, chicken, dog | species reactivity guinea pig, porcine, bovine (proinsulin), rat, monkey, sheep, dog, horse, human (insulin ), rabbit | species reactivity bovine, human, chicken, mouse |
| antibody form purified immunoglobulin | antibody form purified from hybridoma cell culture | antibody form purified immunoglobulin | antibody form ascites fluid |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| clone hVIN-1, monoclonal | clone VIN-11-5, monoclonal | clone K36AC10, monoclonal | clone VIN-11-5, monoclonal |
| Gene Information human ... VCL(7414) | Gene Information mouse ... Vcl(22330) | Gene Information bovine ... Ins(280829) | Gene Information human ... VCL(7414) |
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Classe di stoccaggio
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Hi, what is the recommended secondary for western blot with this antibody? Thanks
1 risposta-
Utile?
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Hallo, in welcher Verdünnung kann der Antikörper zu Western Blot-Zwecken verwendet werden? Vielen Dank im Voraus!
1 risposta-
This product has been determined to be suitable for western blot analysis at a concentration of 0.05-0.1 μg/mL using HS-68 Human foreskin fibroblast cell extract. The working concentration with this material is tested on a lot-to-lot basis.
A sample CofA is available at this link: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/V9264-25UL0000247701.pdf
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