V9131

Anti-Vinculin Antibody

mouse monoclonal, hVIN-1

• Synonym(s): Anti Vinculin, Vinculin Antibody, Vinculin Antibody - Monoclonal Anti-Vinculin antibody produced in mouse
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00162905
• Conjugate: unconjugated
• Clone: hVIN-1, monoclonal
• Application: IF, IHC (f), WB
• Citations: 2013

Properties

• Product Name: Monoclonal Anti-Vinculin antibody produced in mouse, clone hVIN-1, ascites fluid
• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: hVIN-1, monoclonal
• mol wt: antigen 116 kDa
• contains: 15 mM sodium azide
• species reactivity: frog, chicken, mouse, canine, human, bovine, rat, turkey
• enhanced validation: independent; Learn more about Antibody Enhanced Validation
• technique(s): immunohistochemistry (frozen sections): suitable, indirect immunofluorescence: 1:400 using cultured human fibroblasts, western blot: 1:200 using extract of human fibroblasts
• isotype: IgG1
• UniProt accession no.: P18206
• application(s): research pathology
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... VCL(7414) ; mouse ... Vcl(22330) ; rat ... Vcl(305679)

Description

General description

Monoclonal Anti-Vinculin (mouse IgG1 isotype) is derived from the hVIN-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletal muscle), neuromuscular junctions and the membrane-associated dense bodies of smooth muscle. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.

Immunogen

purified human vinculin from uterus.

Application

Monoclonal Anti-Vinculin antibody produced in mouse has been used in:

• western blotting
• immunofluorescence staining
• immunohistochemistry

Biochem/physiol Actions

Monoclonal Anti-Vinculin specifically stains vinculin at cell-cell and cell-substrate contacts in tissue and cultured cells using indirect immunofluorescent labeling. The antibody reacts with the 116 kDa vinculin band in immunoblotting. The product reacts with vinculin of many species. Good reactivity is obtained with human, bovine, chicken, dog, rat, mouse, turkey, and Xenopus. The antibody shows cross reactivity with smooth muscle metavinculin.

Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin.

Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. A sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the β-subunit of integrin binds to talin. Talin binds to vinculin that interacts with α-actinin and possibly with itself. Since α-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system.

Other Notes

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Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable