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V9131

Sigma-Aldrich

Monoclonal Anti-Vinculin antibody produced in mouse

enhanced validation

clone hVIN-1, ascites fluid

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Synonym(s):
Anti Vinculin, Vinculin Antibody, Vinculin Antibody - Monoclonal Anti-Vinculin antibody produced in mouse
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

hVIN-1, monoclonal

mol wt

antigen 116 kDa

contains

15 mM sodium azide

species reactivity

frog, chicken, mouse, canine, human, bovine, rat, turkey

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunohistochemistry (frozen sections): suitable
indirect immunofluorescence: 1:400 using cultured human fibroblasts
western blot: 1:200 using extract of human fibroblasts

isotype

IgG1

UniProt accession no.

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)

General description

Monoclonal Anti-Vinculin (mouse IgG1 isotype) is derived from the hVIN-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletal muscle), neuromuscular junctions and the membrane-associated dense bodies of smooth muscle. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.

Specificity

Monoclonal Anti-Vinculin specifically stains vinculin at cell-cell and cell-substrate contacts in tissue and cultured cells using indirect immunofluorescent labeling. The antibody reacts with the 116 kDa vinculin band in immunoblotting. The product reacts with vinculin of many species. Good reactivity is obtained with human, bovine, chicken, dog, rat, mouse, turkey, and Xenopus. The antibody shows cross reactivity with smooth muscle metavinculin.
Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin.

Immunogen

purified human vinculin from uterus.

Application

Monoclonal Anti-Vinculin antibody produced in mouse has been used in:
  • western blotting
  • immunofluorescence staining
  • immunohistochemistry

Biochem/physiol Actions

Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. A sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the β-subunit of integrin binds to talin. Talin binds to vinculin that interacts with α-actinin and possibly with itself. Since α-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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