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A0162

Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−AP

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0.25 mL
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$149.00
0.5 mL
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$227.00
1 mL
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$411.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162696
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
ELISA (d), WB
Citations:
17

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biological source

goat

Quality Segment

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

technique(s)

direct ELISA: 1:3,000 using IgG, IgA, IgM, western blot: 1:50 dilution

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Immunoglobulins are proteins produced by B cells in response to antigen and regulate response to bacteria and viruses. IgG is known to regulate complement fixation and placental transport. IgA has a crucial role in mucosal immunity as it restricts pathogens from entering the mucosal membrane. IgM is the largest antibody having a pentameric structure which modulates polyreactivity and removes apoptotic cells.

Application

Alkaline-phosphatase-conjugated anti-mouse polyvalent immunoglobulin was used to detect antibodies from hybridomas by indirect ELISA. The antibody was incubated in microtiter plates for 1 hour at 37°C and detected using p-nitrophenyl phosphate substrate (Sigma). Alkaline-phosphatase-conjugated anti-mouse polyvalent immunoglobulin was used to detect the reactivity of fungal cytoplasmic protein with proteins from the serum of mice infected with yeast-form Y cells by western blot analysis. The antibody was used at a 1:50 dilution.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
A3313I8010A2306
Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

A0162

Anti-Mouse Polyvalent Immunoglobulins (G,A,M)−Alkaline Phosphatase antibody produced in goat

Anti-Human Polyvalent Immunoglobulins (α, γ and μ-chain specific)−Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A3313

Anti-Human Polyvalent Immunoglobulins (α, γ and μ-chain specific)−Alkaline Phosphatase antibody produced in goat

Anti-Human Polyvalent Immunoglobulins (G,A,M) antibody produced in rabbit whole antiserum

Sigma-Aldrich

I8010

Anti-Human Polyvalent Immunoglobulins (G,A,M) antibody produced in rabbit

Monoclonal Anti-Rabbit Immunoglobulins−Alkaline Phosphatase antibody produced in mouse clone RG-16, purified immunoglobulin, buffered aqueous glycerol solution

Sigma-Aldrich

A2306

Monoclonal Anti-Rabbit Immunoglobulins−Alkaline Phosphatase antibody produced in mouse

description

affinity isolated antibody, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous glycerol solution

description

whole antiserum

description

clone RG-16, purified immunoglobulin, buffered aqueous glycerol solution

technique(s)

direct ELISA: 1:3,000 using IgG, IgA, IgM, western blot: 1:50 dilution

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

immunoelectrophoresis: suitable

technique(s)

direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160 using human tonsil and Cat. No. I8635 as the primary antibody, western blot: 1:160,000-1:320,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per well)

biological source

goat

biological source

goat

biological source

rabbit

biological source

mouse

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

unconjugated

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

whole antiserum

antibody form

purified immunoglobulin

species reactivity

mouse

species reactivity

human

species reactivity

-

species reactivity

rabbit

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

-

form

buffered aqueous glycerol solution

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Storage Class

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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