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Monoclonal Anti-Biotin−Peroxidase antibody produced in mouse

Name: Monoclonal Anti-Biotin−Peroxidase antibody produced in mouse
Brand: SIGMA
Price: 379 USD
Availability: InStock

clone BN-34, purified immunoglobulin

Synonym(s):

Monoclonal Anti-Biotin

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Pack Size	SKU	Availability	Price
1 vial		Available to ship TODAYfromMILWAUKEE	$379.00

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

peroxidase conjugate

Clone:

BN-34, monoclonal

Application:

DB, ELISA (i), FACS, IHC (p), WB CL

Citations:

$379.00

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Properties

Quality Level

200

biological source

mouse

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

BN-34, monoclonal

packaging

vial of 0.5 mL

technique(s)

dot blot: suitable, flow cytometry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300, indirect ELISA: 1:30,000, western blot (chemiluminescent): 1:80,000

isotype

IgG1

storage temp.

2-8°C

target post-translational modification

unmodified

Description

General description

The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immunohistochemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.

Monoclonal Anti-Biotin (mouse IgG1 isotype) has been derived from the BN-34 hybridoma produced by the used successfully for detection of micro-injected, fusion of mouse myeloma cells and splenocytes from biotin-haptenized cytoskeletal proteins which enable BALB/c mice immunized with biotinylated keyhole limpet hemocyanin (KLH).

Immunogen

Biotin-KLH conjugate

Application

May be substituted for avidin to decrease non-specific binding.

Monoclonal Anti-Biotin Peroxidase antibody produced in mouse has been used:

in immunohistochemistry
in western blot
as secondary antibody to detect the presence of aptamer on the membrane

The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

Biochem/physiol Actions

Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays for detecting antigen-antibody interactions. The use of monoclonal anti-biotin IgG can enhance the sensitivity of such avidin-biotin immunoassays. The monoclonal antibody functions by selectively enlarging and bridging the avidin-biotin-enzyme complexes, thereby increasing the signals from substrate conversion. Furthermore, this antibody can be used in many other applications where biotin may be introduced as target label.
Monoclonal Anti-Biotin-Peroxidase antibody specific for biotin conjugated to various proteins. The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immunohistochemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. The product specificity has been evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	C5585	A1559	F4024
Sigma-Aldrich A0185 Monoclonal Anti-Biotin−Peroxidase antibody produced in mouse	Sigma-Aldrich C5585 Monoclonal Anti-Biotin–Cy3^™ antibody produced in mouse	Sigma-Aldrich A1559 Monoclonal Anti-Biotin–Agarose antibody produced in mouse	Sigma-Aldrich F4024 Anti-Biotin–FITC antibody, Mouse monoclonal
conjugate peroxidase conjugate	conjugate CY3 conjugate	conjugate agarose conjugate	conjugate FITC conjugate
biological source mouse	biological source mouse	biological source mouse	biological source mouse
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture
clone BN-34, monoclonal	clone BN-34, monoclonal	clone BN-34, monoclonal	clone BN-34, monoclonal
isotype IgG1	isotype IgG1	isotype IgG1	isotype IgG1
technique(s) dot blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300, western blot (chemiluminescent): 1:80,000, flow cytometry: suitable, indirect ELISA: 1:30,000	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400, indirect immunofluorescence: 1:200	technique(s) ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80

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pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000508372

0000410508

0000090788

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Global Trade Item Number

SKU	GTIN
A0185-1VL	04061833327098

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