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A0349

Anti-l-Afadin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Size/SKUAvailabilityPrice
0.2 mL
Please contact Customer Service for Availability
6 390,00 kr

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD03455360
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IF, IHC (f), WB
Citations:
22

6 390,00 kr

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biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~200 kDa

species reactivity

canine, mouse, rat, human

packaging

antibody small pack of 25 μL

enhanced validation

independent
Learn more about Antibody Enhanced Validation

technique(s)

immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections., indirect immunofluorescence: 1:500 using MDCK cells and cultured human HepG2 cells., microarray: suitable, western blot: 1:2,000 using extract of rat brain

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MLLT4(4301)
mouse ... Mllt4(17356)
rat ... Mllt4(26955)

General description

Afadin is a multidomain protein that binds F-actin and E-catenin proteins at cell-cell adheren junctions. I-afadin is a ubiquitously expressed splice variant of afadin that contains PDZ and F-actin binding domains at the C-terminal end. I-afadin is homologous to the human AF-6 protein .

Immunogen

Synthetic peptide sequence corresponding to amino acid residues 1814-1829 of rat afadin with N-terminal cysteine conjugated to maleimide-activated KLH.

Application

Anti-l-Afadin antibody produced in rabbit has been used in:
  • western blotting
  • immunofluorescence experiments
  • immunocytochemistry
  • immunohistochemistry

Biochem/physiol Actions

AF-6/Afadin regulates the formation of tight and adherens junctions in the cell and its absence in mice leads to embryonic defects and lethality . Afadin is implicated in primary breast cancer and pancreatic cancer. It downregulates the process of migration and metastasis in cancer cells.
Anti-l-Afadin antibody is specific for I-afadin (approximately 200 kDa). It also binds I-afadin in rat, mouse, dog and human.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-l-Afadin antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

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antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

technique(s)

immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections., microarray: suitable, indirect immunofluorescence: 1:500 using MDCK cells and cultured human HepG2 cells., western blot: 1:2,000 using extract of rat brain

technique(s)

immunocytochemistry: suitable, immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections., indirect immunofluorescence: 1:250 using cultured dog (MDCK) cells and of cultured human HepG2 cells, indirect immunofluorescence: 1:500 using frozen sections of mouse liver, microarray: suitable, western blot: 1:1000 using extract of rat brain.

technique(s)

immunohistochemistry: suitable using suitable for IHC on rat brain perfused with 4% paraformaldehyde, microarray: suitable, western blot: 0.5-1.0 μg/mL using mouse brain and rat brain extract (S1 fraction)

technique(s)

immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:200-1:500

form

buffered aqueous solution

form

-

form

buffered aqueous solution

form

buffered aqueous glycerol solution

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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