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Anti-l-Afadin antibody produced in rabbit

Name: Anti-l-Afadin antibody produced in rabbit
Brand: SIGMA
Price: 599 USD
Availability: InStock

Enhanced Validation

affinity isolated antibody, buffered aqueous solution

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Size/SKU	Availability	Price
0.2 mL	Check Cart for Availability	$599.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD03455360

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ARR, IF, IHC (f), WB

Citations:

$599.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~200 kDa

species reactivity

canine, mouse, rat, human

packaging

antibody small pack of 25 μL

enhanced validation

independent
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technique(s)

immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections., indirect immunofluorescence: 1:500 using MDCK cells and cultured human HepG2 cells., microarray: suitable, western blot: 1:2,000 using extract of rat brain

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MLLT4(4301)
mouse ... Mllt4(17356)
rat ... Mllt4(26955)

Description

General description

Afadin is a multidomain protein that binds F-actin and E-catenin proteins at cell-cell adheren junctions. I-afadin is a ubiquitously expressed splice variant of afadin that contains PDZ and F-actin binding domains at the C-terminal end. I-afadin is homologous to the human AF-6 protein .

Immunogen

Synthetic peptide sequence corresponding to amino acid residues 1814-1829 of rat afadin with N-terminal cysteine conjugated to maleimide-activated KLH.

Application

Anti-l-Afadin antibody produced in rabbit has been used in:

western blotting
immunofluorescence experiments
immunocytochemistry
immunohistochemistry

Biochem/physiol Actions

AF-6/Afadin regulates the formation of tight and adherens junctions in the cell and its absence in mice leads to embryonic defects and lethality . Afadin is implicated in primary breast cancer and pancreatic cancer. It downregulates the process of migration and metastasis in cancer cells.

Anti-l-Afadin antibody is specific for I-afadin (approximately 200 kDa). It also binds I-afadin in rat, mouse, dog and human.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A0224	M3567	M8064
Sigma-Aldrich A0349 Anti-l-Afadin antibody produced in rabbit	Sigma-Aldrich A0224 Anti-l/s-Afadin antibody produced in rabbit	Sigma-Aldrich M3567 Anti-Myosin Iβ (Nuclear) antibody produced in rabbit	Sigma-Aldrich M8064 Anti-Myosin IIA, non muscle antibody produced in rabbit
description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
species reactivity canine, mouse, rat, human	species reactivity rat, human, canine, mouse	species reactivity human, mouse, canine, rat	species reactivity rat, human, canine

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c