Key Documents

Anti-ATG5 (C-terminal) antibody produced in rabbit

Name: Anti-ATG5 (C-terminal) antibody produced in rabbit
Brand: SIGMA
Price: 730 CAD

~1 mg/mL, affinity isolated antibody

Synonym(s):

Anti-Atg5 (C-terminal) antibody produced in rabbit, Anti-ASP, Anti-Apg5, Anti-Apg5L, Anti-Apoptosis-specific protein, Anti-Autophagy protein 5-like

Select a Size

Change View

Size/SKU	Availability	Price
100 μL	Please contact Customer Service for Availability	$730.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD09265351

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ICC, WB

Citations:

$730.00

Please contact Customer Service for Availability

A recombinant, preservative-free antibody is available for your target. Try ZRB1596

Request a Bulk Order

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

mol wt

antigen ~56 kDa

species reactivity

mouse, human

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunocytochemistry: suitable, western blot: 1-2 μg/mL using whole extract of mouse liver or human U87 cells

UniProt accession no.

Q9H1Y0

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ATG5(9474)
mouse ... Atg5(11793)

Description

General description

ATG5 (autophagy related 5) is located on human chromosome band 6q21.

ATG5 was found localized to double FYVE domain containing protein 1 (DFCP1)- specific omegasomes. [1]

Macroautophagy, usually referred to as autophagy, is a major pathway for bulk degradation of cytoplasmic constituents and organelles. Autophagy is an evolutionary conserved pathway seen in all eukaryotic cells. At least 16 ATG genes, required for autophagosome formation were identified in yeast by genetic screens. For many of these genes, related homologs have been identified in mammals. Atg5, also known as Apg5, is a 32 kDa protein essential for autophagy. Atg5 is covalently modified by Atg12, a ubiquitin-like modifier. This conjugation reaction requires ATP and two enzymes, Atg7 and Atg10, which are E1- and E2-like enzymes, respectively. The Atg12-Atg5 conjugate interacts non-covalently with Atg16. The Atg12-Atg5-Atg16 complex localizes to autophagosome precursors and plays an essential role in autophagosome formation. It has been reported that in addition to the role of Atg5 in the formation of autophagosomes, an Atg5 fragment produced by calpain cleavage has pro-apoptotic properties.

Rabbit polyclonal anti-ATG5 (C-terminal) antibody recognizes mouse and human Atg5-Atg12 complex by immunoblotting, ~56 kDa. Detection of the Atg5-Atg12 complex band by immunoblotting is specifically inhibited with the immunizing peptide.

Immunogen

synthetic peptide corresponding to amino acids 262-275 of mouse Atg5, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence differs by one amino acid in human.

Application

Anti-ATG5 (C-terminal) antibody has been used in immunohistochemical (IHC) staining.

Rabbit anti-ATG5 (C-terminal) antibody has been used for western blot analysis in U2OS cells. The antibody has also been used for immunoblot assays in CT26 and MCA205 cells.

Rabbit polyclonal anti-ATG5 (C-terminal) antibody is used to tag Atg5-Atg12 complex for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of Atg5-Atg12 complex in the formation and function of autophagosomes. Rabbit anti-ATG5 was used as primary antibody for immunocytochemistry on HEK293 and Cos7 cells. Cells were fixed in 3.7% formaldehyde in 200 mM Hepes prior to antibody treatment.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	A0856	A2856	A2859
Sigma-Aldrich A0731 Anti-ATG5 (C-terminal) antibody produced in rabbit	Sigma-Aldrich A0856 Anti-ATG5 (N-terminal) antibody produced in rabbit	Sigma-Aldrich A2856 Anti-ATG7 antibody produced in rabbit	Sigma-Aldrich A2859 Monoclonal Anti-ATG5 antibody produced in mouse
biological source rabbit	biological source rabbit	biological source rabbit	biological source mouse
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form purified immunoglobulin
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

Still not finding the right product?

Try our Product Selector Tool to narrow your options

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.