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A0793

Anti-Dog IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Rabbit Anti-Dog IgG (whole molecule)–AP

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1 mL
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€481.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00162265
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d), WB CL
Citations:
8

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biological source

rabbit

Quality Segment

200

conjugate

alkaline phosphatase conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

dog

technique(s)

direct ELISA: 1:6,000, dot blot: 1:4,000, western blot (chemiluminescent): 1:4,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. In dogs four subclasses of IgG (IgG1 -IgG4) are present.
Dog IgGs are glycoprotein antibodies that regulate several immunological responses. Canine IgGs can be used as markers for detecting immune-mediated haemolytic anaemia, adverse food reactions and also for assessing the risk of L. infantum transmission in dogs.

Immunogen

IgG isolated from pooled normal dog serum

Application

Anti-Dog IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA), and immunoblotting.

Biochem/physiol Actions

Anti-Dog IgG (whole molecule) antibody is specific for IgG in dogs when tested against normal dog serum and dog IgG by double-diffusion and immunoelectrophoretic assays prior to conjugation.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Dog IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

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Anti-Bovine IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit affinity isolated antibody

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Anti-Bovine IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

conjugate

alkaline phosphatase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

species reactivity

dog

species reactivity

-

species reactivity

-

species reactivity

bovine

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

technique(s)

direct ELISA: 1:6,000, western blot (chemiluminescent): 1:4,000, dot blot: 1:4,000

technique(s)

direct ELISA: 1:25,000, dot blot: 1:40,000 using direct chemiluminescence assay using 10 ng dog IgG/dot. Luminol plus enhancer was used as substrate.

technique(s)

direct ELISA: 1:10,000

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

-

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flash_point_f

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flash_point_c

Not applicable

Storage Class

12 - Non Combustible Liquids

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