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Bovine Serum Albumin

Name: Bovine Serum Albumin
Brand: SIGMA
Price: 12200 JPY
Availability: InStock

chromatographically purified, New Zealand origin, low endotoxin, suitable for cell culture, pH 7, ≥98%

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

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Size/SKU	Availability	Price
1 g	Available to ship TODAYfrom市川市大町368	¥12,200
5 g	Available to ship TODAYfrom市川市大町368	¥38,400
25 g	Available to ship TODAYfrom市川市大町368	¥130,000
100 g	Check Cart for Availability	¥357,000

About This Item

CAS Number:

9048-46-8

UNSPSC Code:

12352202

EC Number:

232-936-2

NACRES:

NA.27

MDL number:

MFCD00130384

eCl@ss:

32160409

Biological source:

bovine

Assay:

≥98%

Form:

lyophilized powder

Technique(s):

cell culture | mammalian: suitable

Impurities:

≤1 EU/mg endotoxin

¥12,200

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Properties

biological source

bovine

Quality Segment

200

product line

BioReagent

assay

≥98%

form

lyophilized powder

purified by

chromatography

packaging

poly bottle of

origin

New Zealand origin

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/mg endotoxin

pH

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Description

General description

Bovine serum albumin (BSA) is a 66 kDa globular protein crucial for transporting molecules like fatty acids, drugs and hormones from the bloodstream. BSA is a drug carrier for bioactive compounds and has wide clinical applications. BSA has extensive usage in biological research including cell culture, molecular biology, protein biochemistry, and detection techniques. It has wide pharmaceutical applications due to ease of production and interaction flexibility.

Application

Bovine serum albumin has been used:

as a standard in bradford protein estimation method in yeast cells
in elispot assay of blood cells
as a blocking agent in immunohistochemical studies of transplant
as a blocking agent in western blotting of human gastric cancer cell lines

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

Tested for use in cell culture
Low endotoxin

Preparation Note

Prepared by salt fractionation, ion exchange, and gel filtration chromatography.

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

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1 of 1

This Item	A8806	A4919	A0281
Sigma-Aldrich A1933 Bovine Serum Albumin	Sigma-Aldrich A8806 Bovine Serum Albumin	$Bovine Serum Albumin heat shock fraction, protease free, low endotoxin, suitable for cell culture, pH 7, ≥98%$ Sigma-Aldrich A4919 Bovine Serum Albumin	Sigma-Aldrich A0281 Bovine Serum Albumin
biological source bovine	biological source bovine	biological source bovine	biological source bovine
origin New Zealand origin	origin USA origin	origin USA origin	origin USA origin
assay ≥98%	assay ≥96% (agarose gel electrophoresis)	assay ≥98%	assay ≥99% (agarose gel electrophoresis)
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) enzyme immunoassay: suitable
Quality Level 200	Quality Level 300	Quality Level 200	Quality Level 300
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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