This product is a polyclonal antibody. Polyclonal antibodies are heterologous mixtures of IgG isotypes.
A2052
Anti-GABA antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Promotion Available
Synonym(s):
Anti Gaba, Anti Gaba Antibody, Anti-Gaba, GABA Antibody - Anti-GABA antibody produced in rabbit, Gaba Antibody, Anti-γ-Aminobutyric acid
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| Size/SKU | Availability | Price |
|---|---|---|
25 μL | Check Cart for Availability | $261.00 |
100 μL | Check Cart for Availability | $756.00 |
0.2 mL | Please contact Customer Service for Availability | $1,170.00 |
About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
DB, IHC (p)
Citations:
656
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
rat, Drosophila, wide range
packaging
antibody small pack of 25 μL
technique(s)
dot blot: 1:10,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5 μg/mL using rat cerebellum
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
rat ... Gabra1(29705)
General description
Anti-GABA is produced in rabbit using GABA-BSA as the immunogen. The antibody is isolated from antiserum by immunospecific methods of purification. Antigen specific affinity isolation removes essentially all rabbit serum proteins, including immunoglobulins which do not specifically bind to GABA.
GABA (γ-aminobutyric acid) is a multifunctional molecule found in several organisms, from prokaryotes to vertebrates. It is present in non-nervous structures, such as peripheral nervous and endocrine systems. GABA is formed following decarboxylation of glutamic acid by the enzyme glutamic acid decarboxylase (GAD).
GABA (γ-aminobutyric acid) is a multifunctional molecule found in several organisms, from prokaryotes to vertebrates. It is present in non-nervous structures, such as peripheral nervous and endocrine systems. GABA is formed following decarboxylation of glutamic acid by the enzyme glutamic acid decarboxylase (GAD).
Immunogen
γ-aminobutyric acid (GABA) conjugated to BSA.
Application
Expression of GABA in neucortical cells harvested from the brains of E19 day old rat embyros was detected by immunofluorescence using rabbit anti-GABA antibody. Triple IF staining was performed with the anti-GABA antibody and two anti-GAD antibodies. Expression of GABA was analyzed in cells isolated from the pallium of various animals including rats, mice, rabbits, guinea pigs, and lizards by immunohistochemistry. IHC was performed using rabbit anti-GABA antibody at 1:1000 diluted in a solution of 0.01M PBS ph 7.4 + 0.5% triton-x100.
Rabbit anti-GABA antibody has been used for electron microscopy analysis in rat hippocampal tissues at a dilution of 1:4000. The antibody has also been used for immunocytochemistry applications at dilutions ranging from 1:100-1:750 in Drosophila and Periplaneta americana brain cells.
Biochem/physiol Actions
GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the central nervous system and also functions as a neuromodulator in some peripheral tissues. It controls heart rate, blood pressure and respiratory process. GABA facilitates neuronal inhibition by interacting with GABA/benzodiazepine receptor and opening an integral chloride channel. Rabbit anti-GABA antibody shows positive binding with GABA and GABA-keyhole limpet hemocyanin (KLH) in a dot blot assay, and exhibits negative binding with bovine serum albumin (BSA).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| description affinity isolated antibody, buffered aqueous solution | description clone GB-69, ascites fluid | description clone BD17, Chemicon®, from mouse | description clone KC4-8A7, from mouse |
| biological source rabbit | biological source mouse | biological source mouse | biological source mouse |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| antibody form affinity isolated antibody | antibody form ascites fluid | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| storage temp. −20°C | storage temp. −20°C | storage temp. - | storage temp. - |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in wet ice |
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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whats the sequence of recognition of the antibody
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The peptide sequence for this antibody has not been determined.
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