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Bovine Serum Albumin

Name: Bovine Serum Albumin
Brand: SIGMA
Price: 77.1 USD
Availability: InStock

lyophilized powder, essentially IgG-free, low endotoxin, BioReagent, suitable for cell culture

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

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Size/SKU	Availability	Price
1 g	Check Cart for Availability	$77.10
5 g	Check Cart for Availability	$253.00
25 g	Check Cart for Availability	$860.00
100 g	Check Cart for Availability	$2,710.00

About This Item

CAS Number:

9048-46-8

UNSPSC Code:

12352202

EC Number:

232-936-2

NACRES:

NA.27

MDL number:

MFCD00130384

eCl@ss:

32160409

Biological source:

bovine

Assay:

≥95% (agarose gel electrophoresis)

Form:

lyophilized powder

Technique(s):

cell culture | mammalian: suitable

Impurities:

IgG free, ≤0.05% IgG, ≤1 EU/mg endotoxin

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Properties

biological source

bovine

Quality Segment

300

product line

BioReagent

assay

≥95% (agarose gel electrophoresis)

form

lyophilized powder

purified by

chromatography

packaging

poly bottle of

origin

New Zealand origin

technique(s)

cell culture | mammalian: suitable

impurities

IgG free, ≤0.05% IgG, ≤1 EU/mg endotoxin

pH

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Description

General description

Bovine serum albumin (BSA) is a non-glycosylated protein belonging to the serum albumins family. It is a globular protein with α-helix structure and 17-disulfide bonds. BSA has a molecular weight of 66 kDa consisting three domains and two subdomains under each. BSA shows structural similarity with human serum albumin.

Application

Bovine Serum Albumin has been used to treat polyomavirus BK to serve as a control in testing intravenous immunoglobulins preparations.

BSA is widely used as a blocking agent in immunoassays and immunodetection procedures, and also as a carrier protein for dilution of antibodies. Thus it is of concern to be aware of potential trace endogenous content of globulins / IgG in BSA, because of concerns that such trace globulins / IgG may serve as antigens for secondary antibodies in immunodetection work. This product undergoes testing for low IgG content / status as essentially immunoglobulin-free.

Select the right albumin for your application with the Albumin Selection Guide

Biochem/physiol Actions

Bovine Serum Albumin (BSA) acts as a carrier protein for drugs, hormones and fatty acids. It is an essential component of cell culture medium. BSA serves as a blocking agent in enzyme linked immunosorbent assay (ELISA) and prevents non-specific binding of antigens and antibodies to the microtiter plates. Due to its high solubility, low cost and purity and interaction with surfactant, it is useful in pharmaceutical and cosmetic industry.

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

Tested for use in cell culture
Low endotoxin
Essentially IgG-free (immunoglobulin free)

Preparation Note

Prepared by salt fractionation, ion exchange, and gel filtration chromatography.

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

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This Item	A3059	A1933	A3294
Sigma-Aldrich A2058 Bovine Serum Albumin	$Bovine Serum Albumin heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%$ Sigma-Aldrich A3059 Bovine Serum Albumin	Sigma-Aldrich A1933 Bovine Serum Albumin	$Bovine Serum Albumin heat shock fraction, protease free, pH 7, ≥98%$ Sigma-Aldrich A3294 Bovine Serum Albumin
description lyophilized powder, essentially IgG-free, low endotoxin, BioReagent, suitable for cell culture	description heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%	description chromatographically purified, New Zealand origin, low endotoxin, suitable for cell culture, pH 7, ≥98%	description heat shock fraction, protease free, pH 7, ≥98%
biological source bovine	biological source bovine	biological source bovine	biological source bovine
origin New Zealand origin	origin USA origin	origin New Zealand origin	origin USA origin
assay ≥95% (agarose gel electrophoresis)	assay ≥98%	assay ≥98%	assay ≥98%
technique(s) cell culture \| mammalian: suitable	technique(s) ELISA: suitable, western blot: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) microbiological culture: suitable, blood typing: suitable, ELISA: suitable
Quality Level 300	Quality Level 300	Quality Level 200	Quality Level 300
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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