Key Documents

Monoclonal Anti-Rabbit Immunoglobulins–Peroxidase antibody produced in mouse

Name: Monoclonal Anti-Rabbit Immunoglobulins–Peroxidase antibody produced in mouse
Brand: SIGMA
Price: 426.7 USD
Availability: InStock

clone RG-16, purified immunoglobulin, lyophilized powder

Synonym(s):

Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)

Select a Size

Change View

Size/SKU	Availability	Price
1 vial	Available to ship TODAYfromMILWAUKEE	$502.00 $426.70

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

peroxidase conjugate

Clone:

RG-16, monoclonal

Application:

ELISA (d), IHC (p), WB CL

Citations:

$426.70

List Price$502.00Save 15%

Web-Only Promotion

Available to ship TODAYDetails

Request a Custom Order

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

mouse

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

RG-16, monoclonal

form

lyophilized powder

species reactivity

rabbit

should not react with

human, pig, chicken, bovine, horse, guinea pig, turkey, rat, canine, goat, feline, sheep

packaging

vial of 0.5 mL

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissue, western blot (chemiluminescent): 1:160,000

isotype

IgG1

storage temp.

2-8°C

target post-translational modification

unmodified

Description

General description

Monoclonal Anti-Rabbit Immunoglobulins (mouse IgG1 isotype) is derived from the RG-16 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rabbit IgG. Immunoglobulins (Igs) belongs to the immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L) chains held together by disulfide linkages. Each light comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. IgG is abundant immunoglobulin in human serum. The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4.

Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.

Application

Monoclonal Anti-Rabbit Immunoglobulins-Peroxidase antibody produced in mouse has been used in:

western blotting
immunoblotting
immunoprecipitation
enzyme linked immunosorbent assay (ELISA)
immunohistochemistry

Mouse monoclonal anti-rabbit immunoglobulins have been used for western blot assays at a dilution of 1:1000 using bovine trabecular meshwork cell lysates. The antibody can also be used for immunohistochemistry assays (1:100, using human tissue).

The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.

Biochem/physiol Actions

Binds only rabbit Igs. Does not react with reduced rabbit Igs.

Physical form

Lyophilized from 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	A1949	A2306	A2556
Sigma-Aldrich A2074 Monoclonal Anti-Rabbit Immunoglobulins–Peroxidase antibody produced in mouse	Sigma-Aldrich A1949 Anti-Rabbit IgG (γ-chain specific)–Peroxidase antibody, Mouse monoclonal	Sigma-Aldrich A2306 Monoclonal Anti-Rabbit Immunoglobulins−Alkaline Phosphatase antibody produced in mouse	Sigma-Aldrich A2556 Monoclonal Anti-Rabbit IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in mouse
description clone RG-16, purified immunoglobulin, lyophilized powder	description clone RG-96, purified from hybridoma cell culture	description clone RG-16, purified immunoglobulin, buffered aqueous glycerol solution	description clone RG-96, purified immunoglobulin, buffered aqueous glycerol solution
biological source mouse	biological source mouse	biological source mouse	biological source mouse
conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate
species reactivity rabbit	species reactivity rabbit	species reactivity rabbit	species reactivity rabbit
technique(s) western blot (chemiluminescent): 1:160,000, direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissue	technique(s) direct ELISA: 1:40,000, western blot: 1:160,000 using total cell extract of HeLa cells (5-10 μg/well), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160 using human tonsil and Cat. No. I8635 as the primary antibody, direct ELISA: 1:40,000, western blot: 1:160,000-1:320,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per well)	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, western blot: 1:200,000-1:400,000 using total cell extract of HeLa cells, direct ELISA: 1:50,000
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin
isotype IgG1	isotype IgG1	isotype IgG1	isotype IgG1

Still not finding the right product?

Try our Product Selector Tool to narrow your options

hcodes

H317

pictograms

GHS07

signalword

Warning

pcodes

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 2

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.