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Monoclonal Anti-Rabbit Immunoglobulins–Peroxidase antibody produced in mouse

Name: Monoclonal Anti-Rabbit Immunoglobulins–Peroxidase antibody produced in mouse
Brand: SIGMA
Price: 520 USD
Availability: InStock

clone RG-16, purified immunoglobulin, lyophilized powder

Synonym(s):

Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)

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Size/SKU	Availability	Price
1 vial	Check Cart for Availability	$502.00 $520.00

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

peroxidase conjugate

Clone:

RG-16, monoclonal

Application:

ELISA (d), IHC (p), WB CL

Citations:

$520.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

RG-16, monoclonal

form

lyophilized powder

species reactivity

rabbit

should not react with

human, pig, chicken, bovine, horse, guinea pig, turkey, rat, canine, goat, feline, sheep

packaging

vial of 0.5 mL

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissue, western blot (chemiluminescent): 1:160,000

isotype

IgG1

storage temp.

2-8°C

target post-translational modification

unmodified

Description

General description

Monoclonal Anti-Rabbit Immunoglobulins (mouse IgG1 isotype) is derived from the RG-16 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rabbit IgG. Immunoglobulins (Igs) belongs to the immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L) chains held together by disulfide linkages. Each light comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. IgG is abundant immunoglobulin in human serum. The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4.

Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.

Application

Monoclonal Anti-Rabbit Immunoglobulins-Peroxidase antibody produced in mouse has been used in:

western blotting
immunoblotting
immunoprecipitation
enzyme linked immunosorbent assay (ELISA)
immunohistochemistry

Mouse monoclonal anti-rabbit immunoglobulins have been used for western blot assays at a dilution of 1:1000 using bovine trabecular meshwork cell lysates. The antibody can also be used for immunohistochemistry assays (1:100, using human tissue).

The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.

Biochem/physiol Actions

Binds only rabbit Igs. Does not react with reduced rabbit Igs.

Physical form

Lyophilized from 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	B3275	F4890	A8400
Sigma-Aldrich A2074 Monoclonal Anti-Rabbit Immunoglobulins–Peroxidase antibody produced in mouse	Sigma-Aldrich B3275 Monoclonal Anti-Rabbit Immunoglobulins–Biotin antibody produced in mouse	Sigma-Aldrich F4890 Monoclonal Anti-Rabbit Immunoglobulins–FITC antibody produced in mouse	Sigma-Aldrich A8400 Anti-Human Polyvalent Immunoglobulins (G,A,M)−Peroxidase antibody produced in goat
description clone RG-16, purified immunoglobulin, lyophilized powder	description clone RG-16, purified immunoglobulin, buffered aqueous solution	description clone RG-16, purified immunoglobulin, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution
technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissue, direct ELISA: 1:30,000, western blot (chemiluminescent): 1:160,000	technique(s) direct ELISA: 1:60,000, western blot: 1:200,000-1:400,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,500	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, indirect immunofluorescence: 1:50 using using chicken fibroblast cells and primary antibody produced in rabbit	technique(s) direct ELISA: 1:5,000-1:15,000 using IgG, IgA, IgM
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form affinity isolated antibody
conjugate peroxidase conjugate	conjugate biotin conjugate	conjugate FITC conjugate	conjugate peroxidase conjugate
species reactivity rabbit	species reactivity rabbit	species reactivity -	species reactivity -
biological source mouse	biological source mouse	biological source mouse	biological source goat
isotype IgG1	isotype IgG1	isotype IgG1	isotype -

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Hazard Codes

H317

Pictograms

GHS07

Signal Word

Warning

Precautionary Statements

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 2

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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