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Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase, Mouse monoclonal

Name: Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase, Mouse monoclonal
Brand: SIGMA
Price: 270 USD
Availability: InStock

clone MB-11, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase

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Size/SKU	Availability	Price
0.2 mL	Available to ship TODAYfromMILWAUKEE	$270.00

About This Item

NACRES:

NA.77

UNSPSC Code:

12352203

Conjugate:

alkaline phosphatase conjugate

Clone:

MB-11, monoclonal

Application:

DB, ELISA (d)

Citations:

$270.00

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Properties

biological source

mouse

Quality Segment

300

conjugate

alkaline phosphatase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

secondary antibodies

clone

MB-11, monoclonal

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

direct ELISA: 1:35,000, dot blot: 1:20,000 using 20 ng of human IgM/dot, dot blot: 1:30,000 using 20 ng of human IgM/dot (chemiluminescence)

isotype

IgG2b

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Description

General description

Human IgMs are the initial immunoglobulin isotypes that appear in blood in response to the first exposure to external antigens. IgMs have been implicated in CNS myelin repair and increased secretory IgM levels have been linked to rhino-conjunctivitis . Monoclonal Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody is specific for human IgM. The product does not react with human IgG, IgA or light chains.

Monoclonal Anti-Human IgM (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

Human IgM isolated from pooled normal human serum

Application

Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase antibody purified from hybridoma cell culture has been used in immunocytochemistry and enzyme linked immunosorbent assay (ELISA)

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	I6385	A3275	A9688
Sigma-Aldrich A2189 Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase, Mouse monoclonal	Sigma-Aldrich I6385 Monoclonal Anti-Human IgM (μ-chain specific) antibody produced in mouse	Sigma-Aldrich A3275 Anti-Human IgM (μ-chain specific)−Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A9688 Anti-Mouse IgM (μ-chain specific)–Alkaline Phosphatase antibody produced in goat
description clone MB-11, purified from hybridoma cell culture	description clone MB-11, ascites fluid	description affinity isolated antibody, buffered aqueous glycerol solution	description affinity isolated antibody, buffered aqueous glycerol solution
biological source mouse	biological source mouse	biological source goat	biological source goat
technique(s) dot blot: 1:30,000 using 20 ng of human IgM/dot (chemiluminescence), dot blot: 1:20,000 using 20 ng of human IgM/dot, direct ELISA: 1:35,000	technique(s) indirect ELISA: 1:5,000	technique(s) direct ELISA: 1:7,000-1:21,000	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000, direct ELISA: 1:30,000
conjugate alkaline phosphatase conjugate	conjugate unconjugated	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate
species reactivity human	species reactivity -	species reactivity human	species reactivity mouse
antibody form purified from hybridoma cell culture	antibody form ascites fluid	antibody form affinity isolated antibody	antibody form affinity isolated antibody
isotype IgG2b	isotype IgG2b	isotype -	isotype -

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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