Key Documents

Anti-Human IgG (γ-chain specific), F(ab′)₂ fragment−Peroxidase antibody produced in goat

Name: Anti-Human IgG (γ-chain specific), F(ab′)<SUB>2</SUB> fragment−Peroxidase antibody produced in goat
Brand: SIGMA
Price: 396 USD

affinity isolated antibody, buffered aqueous solution

Select a Size

Change View

Size/SKU	Availability	Price
1 mL	Check Cart for Availability	$396.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162447

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

ELISA (d), WB

Citations:

$396.00

Check Cart for Availability

Request a Custom Order

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

goat

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

direct ELISA: 1:10,000, western blot: suitable

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . Anti-Human IgG (γ-chain specific), (F(ab′)₂) fragment-Peroxidase antibody is specific for human IgG when tested against human IgA, IgG, IgM, Bence Jones κ, and λ myeloma proteins.

Immunogen

Purified human IgG

Application

Anti-Human IgG (γ-chain specific), (F(ab′)₂) fragment-Peroxidase antibody is suitable for use in ELISA and western blot .

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)

Peroxidase-conjugated goat anti-human IgG (Fab specific) antibody was used for western blot and ELISA analysis of yeast cultures genetically modified to produced recombinant Fab fragments. Anti-Human IgG antibody was used at a 1:10,000 dilution in PBS and incubated for 1 hour at room temperature. Binding in ELISA assays was detected with a O-phenylene- diamine substrate solution (Sigma).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	SAB4200791	A5403	A9917
Sigma-Aldrich A2290 Anti-Human IgG (γ-chain specific), F(ab′)₂ fragment−Peroxidase antibody produced in goat	Sigma-Aldrich SAB4200791 Anti-Human IgG (Fab specific)-Peroxidase antibody, Mouse monoclonal	Sigma-Aldrich A5403 Anti-Human IgG (γ-chain specific), F(ab′)₂ fragment−Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A9917 Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat
description affinity isolated antibody, buffered aqueous solution	description clone GG-6, purified from hybridoma cell culture	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution
antibody form affinity isolated antibody	antibody form purified from hybridoma cell culture	antibody form affinity isolated antibody	antibody form affinity isolated antibody
technique(s) direct ELISA: 1:10,000, western blot: suitable	technique(s) direct ELISA: 1:16,000-1:32,000 using 2.5 μg/ml human IgG for coating.	technique(s) direct ELISA: 1:1,000	technique(s) western blot: 1:80,000-1:160,000, direct ELISA: 1:60,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150
conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate alkaline phosphatase conjugate	conjugate peroxidase conjugate
biological source goat	biological source mouse	biological source goat	biological source goat
species reactivity human	species reactivity human	species reactivity -	species reactivity mouse
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C

Still not finding the right product?

Try our Product Selector Tool to narrow your options

Pictograms

GHS07,GHS09

Signal Word

Warning

Hazard Codes

H315,H317,H319,H411

Precautionary Statements

P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

WGK

WGK 3

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.