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Anti-Human IgG (γ-chain specific), F(ab′)₂ fragment−Peroxidase antibody produced in goat

Name: Anti-Human IgG (γ-chain specific), F(ab′)<SUB>2</SUB> fragment−Peroxidase antibody produced in goat
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162447

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

ELISA (d), WB

Citations:

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Properties

biological source

goat

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

direct ELISA: 1:10,000, western blot: suitable

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . Anti-Human IgG (γ-chain specific), (F(ab′)₂) fragment-Peroxidase antibody is specific for human IgG when tested against human IgA, IgG, IgM, Bence Jones κ, and λ myeloma proteins.

Immunogen

Purified human IgG

Application

Anti-Human IgG (γ-chain specific), (F(ab′)₂) fragment-Peroxidase antibody is suitable for use in ELISA and western blot .

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)

Peroxidase-conjugated goat anti-human IgG (Fab specific) antibody was used for western blot and ELISA analysis of yeast cultures genetically modified to produced recombinant Fab fragments. Anti-Human IgG antibody was used at a 1:10,000 dilution in PBS and incubated for 1 hour at room temperature. Binding in ELISA assays was detected with a O-phenylene- diamine substrate solution (Sigma).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A6029	A3312	A0170
Sigma-Aldrich A2290 Anti-Human IgG (γ-chain specific), F(ab′)₂ fragment−Peroxidase antibody produced in goat	Sigma-Aldrich A6029 Anti-Human IgG (γ-chain specific)−Peroxidase antibody produced in goat	Sigma-Aldrich A3312 Anti-Human IgG (γ-chain specific), F(ab′)₂ fragment−Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A0170 Anti-Human IgG (Fc specific)−Peroxidase antibody produced in goat
description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous glycerol solution	description affinity isolated antibody
species reactivity human	species reactivity human	species reactivity human	species reactivity human
technique(s) direct ELISA: 1:10,000, western blot: suitable	technique(s) direct ELISA: 1:10,000	technique(s) direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000	technique(s) direct ELISA: 1:60,000, dot blot: 1:100,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate alkaline phosphatase conjugate	conjugate peroxidase conjugate
biological source goat	biological source goat	biological source goat	biological source goat
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal

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pictograms

GHS07,GHS09

signalword

Warning

hcodes

H315,H317,H319,H411

pcodes

P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

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