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A2904

Anti-Human Lambda Light Chains (Bound and Free)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

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Synonym(s):

Goat Anti-Human Lambda Light Chains (Bound and Free)−AP

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0.5 mL
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$744.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162527
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
ELISA (d)
Citations:
5

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biological source

goat

Quality Segment

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

direct ELISA: 1:2,000-1:21,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Immunoglobulins are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. Immunoglobulin light chains are the primary protein components of the amyloid fibrillar substance that is present in patients having myeloma-associated amyloidosis . Furthermore, urinary free immunoglobulin light chains can be used for the immunodiagnosis of Bence Jones proteinuria . Anti-Human λ Light Chains (Bound and Free)-Alkaline Phosphatase antibodies are specific for human λ light chains when tested against bound κ and λ proteins (human IgA, IgG, IgM) and free Bence Jones κ and λ myeloma proteins.
Mammalian immunoglobins contain either lambda or kappa light chains.
Alkaline Phosphatase is an enzyme that catalyzes the conversion of chromogenic substrates such as p-nitrophenylphosphate (PNPP); chemiluminescent substrates such as CDP-Star® and fluorogenic substrates such as 4-methylumbelliferyl phosphate (4-MUP) into detectable chromophores, light-emitters or fluorescers, respectively.

Immunogen

λ light chains isolated from Bence Jones urines

Application

Goat polyclonal anti-Human Lambda Light Chains (Bound and Free)-Alkaline Phosphatase antibody may be used to detect human lamba light chain containing immunoglobulins by chromogenic, fluorogenic, and chemiluminescent techniques. Whole cell bacterial ELISAs were performed to detect the light and heavy fragments of monoclonal antibodies against the meningococcal porA protein. The alkaline phosphatase conjugated goat anti-human λ Light Chains IgG was used as the secondary. The reaction was developed using p-nitrophenyl phosphate substrate (Sigma).

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide

Legal Information

CDP-Star is a registered trademark of Tropix, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
A3813A5175A3400
Anti-Human Lambda Light Chains (Bound and Free)−Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A2904

Anti-Human Lambda Light Chains (Bound and Free)−Alkaline Phosphatase antibody produced in goat

Anti-Human Kappa Light Chains (Bound and Free)−Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A3813

Anti-Human Kappa Light Chains (Bound and Free)−Alkaline Phosphatase antibody produced in goat

Anti-Human Lambda Light Chains (Bound and Free)−Peroxidase antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

A5175

Anti-Human Lambda Light Chains (Bound and Free)−Peroxidase antibody produced in goat

Anti-Human IgA (α-chain specific)−Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

A3400

Anti-Human IgA (α-chain specific)−Alkaline Phosphatase antibody produced in goat

description

affinity isolated antibody, buffered aqueous glycerol solution

description

affinity isolated antibody, buffered aqueous glycerol solution

description

affinity isolated antibody, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous solution

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

goat

biological source

goat

biological source

goat

biological source

goat

species reactivity

human

species reactivity

human

species reactivity

-

species reactivity

human

technique(s)

direct ELISA: 1:2,000-1:21,000

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50

technique(s)

dot blot: 1:40,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, direct ELISA: 1:35,000

technique(s)

direct ELISA: 1:7,000-1:21,000

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

buffered aqueous solution

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    SLBC3107
    SLBP4530V
    SLBQ1221V
    043K9165
    020M6012

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