The specification for each lot indicates that 3-4 mg of protein (Goat Anti-Human IgG) is immobilized per mL of resin.
A3316
Anti-Human IgG (Fc Specific)−Agarose antibody produced in goat
affinity isolated antibody, PBS suspension
Synonym(s):
Goat anti-human IgG
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| Size/SKU | Availability | Price |
|---|---|---|
2 mL | Check Cart for Availability | $605.00 |
5 mL | Check Cart for Availability | $948.00 |
About This Item
Conjugate:
agarose conjugate
Clone:
polyclonal
Application:
ODD
Citations:
9
biological source
goat
Quality Segment
conjugate
agarose conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
PBS suspension
species reactivity
human
should not react with
rat, mouse
technique(s)
Ouchterlony double diffusion: suitable
capacity
2 mg/mL, resin binding capacity (human IgG)
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
Goat Anti-Human IgG (Fc Specific)-Agarose antibody is specific for the Fc fragment of human IgG when tested against purified human IgA, IgG, IgM, Fc, and kappa and lambda light chains. No reactivity with mouse or rat IgG is seen by Ouchterlony Double Diffusion (ODD), prior to agarose bead coupling.
Goat Anti-Human IgG (Fc Specific)-Agarose antibody is specific for the Fc fragment of human IgG when tested against purified human IgA, IgG, IgM, Fc, and kappa and lambda light chains. No reactivity with mouse or rat IgG is seen by Ouchterlony Double Diffusion (ODD), prior to agarose bead coupling.
Immunogen
Fc fragment of human IgG.
Application
Anti-Human IgG (Fc Specific)-Agarose antibody produced in goat has also been used to purify galanin 1 (Gala1), 3Gal-substituted P-selectin glycoprotein ligand-1 (PSGL-1)/hIgG1 from supernatants of transfected COS cells.
Goat Anti-Human IgG (Fc Specific)-Agarose antibody is suitable for use in Ouchterlony double diffusion. The antibody has also been used for affinity purification assays.
Immunoprecipitation assays were performed using goat anti-human IgG (Fc specific) crosslinked to agarose beads. The beads were diluted in 1 ml of 5% FBS/DMEM for 2 hours at 4 degrees.
Physical form
Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Other Notes
Antibody adsorbed with mouse and rat IgG
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| description affinity isolated antibody, PBS suspension | description affinity isolated antibody, buffered aqueous glycerol solution | description affinity isolated antibody, buffered aqueous solution | description affinity isolated antibody, buffered aqueous glycerol solution |
| biological source goat | biological source goat | biological source goat | biological source goat |
| species reactivity human | species reactivity human | species reactivity - | species reactivity - |
| technique(s) Ouchterlony double diffusion: suitable | technique(s) direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000 | technique(s) direct ELISA: 1:55,000, dot blot: 1:40,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150 | technique(s) direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, western blot (chemiluminescent): 1:30,000 using β-actin in total cell extract of HiLa cells (5-10 μg per well) |
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody |
| conjugate agarose conjugate | conjugate alkaline phosphatase conjugate | conjugate alkaline phosphatase conjugate | conjugate alkaline phosphatase conjugate |
| clone polyclonal | clone polyclonal | clone polyclonal | clone polyclonal |
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Storage Class
10 - Combustible liquids
wgk
WGK 3
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Hello, could you tell me how much antibody is in those 2 ml?
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