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A3422

Achromopeptidase from bacteria

partially purified powder, ≥20,000 units/mg solid

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Synonym(s):

ACP, Achromopeptidase

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25000 units

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$660.00
50000 units
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$1,210.00

About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Specific activity:
≥20,000 units/mg solid

$660.00

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form

partially purified powder

Quality Segment

specific activity

≥20,000 units/mg solid

mol wt

27 kDa

foreign activity

Collagenase, present

storage temp.

−20°C

Application

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections. Product A3422 is from bacteria and is a partially purified powder containing lactose.
Achromopeptidase from bacteria may be used:
  • in the homogenization step for genomic DNA extraction from microbial biofilm
  • in the lysis of gram-positive strains(
  • for DNA extraction from Nocardia strains

Biochem/physiol Actions

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C.
Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family. It is effective for the lysis of gram positive bacteria compared to lysozyme. Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals.

Physical form

Partially purified powder.

Other Notes

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

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This Item
G0751A7646A3903
description

partially purified powder, ≥20,000 units/mg solid

description

Type H-1, partially purified powder, ≥300,000 units/g solid

description

ATPase ≥60 units/mg protein, lyophilized powder (partially purified)

description

≥98% (TLC), powder

specific activity

≥20,000 units/mg solid

specific activity

≥300,000 units/g solid

specific activity

-

specific activity

-

form

partially purified powder

form

partially purified powder

form

lyophilized powder (partially purified)

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

mol wt

27 kDa

mol wt

-

mol wt

~49 kDa by gel filtration

mol wt

-

foreign activity

Collagenase, present

foreign activity

-

foreign activity

Acid Phosphatase ≤5% of base activity

foreign activity

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


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pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves



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