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Achromopeptidase from bacteria

Name: Achromopeptidase from bacteria
Brand: SIGMA

partially purified powder, ≥20,000 units/mg solid

Synonym(s):

ACP, Achromopeptidase

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.4.21.50 (BRENDA, IUBMB)

MDL number:

MFCD00130333

Specific activity:

≥20,000 units/mg solid

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Properties

form

partially purified powder

Quality Segment

200

specific activity

≥20,000 units/mg solid

mol wt

27 kDa

foreign activity

Collagenase, present

storage temp.

−20°C

Description

Application

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections[1]. Product A3422 is from bacteria and is a partially purified powder containing lactose.

Achromopeptidase from bacteria may be used:

in the homogenization step for genomic DNA extraction from microbial biofilm
in the lysis of gram-positive strains(
for DNA extraction from Nocardia strains

Biochem/physiol Actions

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD₆₀₀= 0.6 over 2 hours at 37 °C.

Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family. It is effective for the lysis of gram positive bacteria compared to lysozyme. Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals.

Physical form

Partially purified powder.

Other Notes

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

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This Item	A3547	P9752	G0751
Sigma-Aldrich A3422 Achromopeptidase from bacteria	Sigma-Aldrich A3547 Achromopeptidase from bacteria	Sigma-Aldrich P9752 Phosphoriboisomerase from spinach	Sigma-Aldrich G0751 β-Glucuronidase from Helix pomatia
description partially purified powder, ≥20,000 units/mg solid	description lyophilized powder, ≥1,000 units/mg solid	description Type I, partially purified powder, ≥40 units/mg protein (biuret)	description Type H-1, partially purified powder, ≥300,000 units/g solid
specific activity ≥20,000 units/mg solid	specific activity ≥1,000 units/mg solid	specific activity ≥40 units/mg protein (biuret)	specific activity ≥300,000 units/g solid
form partially purified powder	form lyophilized powder	form partially purified powder	form partially purified powder
mol wt 27 kDa	mol wt 27 kDa	mol wt -	mol wt -
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
foreign activity Collagenase, present	foreign activity -	foreign activity -	foreign activity -

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pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P342 + P311

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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