The powder form has an assigned shelf life of two years. This product is soluble in water and aqueous buffers. Please see the link below, page two, for solution stability as this is pH-dependent:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/424/461/a3428pis.pdf
A3428
Aprotinin
3-8 TIU/mg solid, lyophilized powder, suitable for cell culture
Synonym(s):
BPTI, Bovine pancreatic trypsin inhibitor, Trasylol, Trypsin inhibitor (basic)
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 mg | Available to ship TODAYfromMILWAUKEE | $233.00 | |
| 25 mg | Available to ship TODAYfromMILWAUKEE | $545.00 | |
| 100 mg | Check Cart for Availability | $1,700.00 | |
| 250 mg | Available to ship TODAYfromMILWAUKEE | $3,710.00 |
About This Item
Empirical Formula (Hill Notation):
C284H432N84O79S7
CAS Number:
Molecular Weight:
6511.44
UNSPSC Code:
12352200
eCl@ss:
32190102
NACRES:
NA.32
EC Number:
232-994-9
MDL number:
Product Name
Aprotinin from bovine lung, lyophilized powder, 3-8 TIU/mg solid, BioReagent, suitable for cell culture
biological source
bovine lung
Quality Level
product line
BioReagent
form
lyophilized powder
specific activity
3-8 TIU/mg solid
mol wt
~6,500
technique(s)
cell culture | mammalian: suitable
solubility
H2O: 5 mg/mL
UniProt accession no.
storage temp.
2-8°C
SMILES string
S1SCC2NC(=O)CNC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C5NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C9N(CCC9)C(=O)CNC(=O)C(NC(=O)C(NC(=O)C%11N(CCC%11)C(=O
InChI key
ZPNFWUPYTFPOJU-UHFFFAOYSA-N
Gene Information
cow ... PTI(404172)
Application
Aprotinin is largely used as an inhibitor of trypsin.
Aprotinin from bovine lung has been used in insulin and glucagon-like peptide-1 assay. It has also been used a in western blotting.[1]
Biochem/physiol Actions
Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.
Aprotinin is a natural polypeptide of 58 amino acids. It possesses hemostatic/antiplasmin action and helps to reduce bleeding during surgeries. This prevents the necessity of blood transfusion.
Preparation Note
This product is a lyophilized powder that has been cell culture tested. It has an activity of 3-7 TIU/mg.
Other Notes
One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.
When benzoyl-L-arginine ethyl ester (BAEE) is used as substrate one BAPNA unit is approx. 45BAEE μmolar units at pH8.0 at 25°C or approx. 9,000 BAEE A253 units at pH7.6 at 25°C.
1 of 1
This Item | |||
|---|---|---|---|
| biological source bovine lung | biological source bovine lung | biological source bovine lung | biological source bovine |
| Gene Information cow ... PTI(404172) | Gene Information cow ... PTI(404172) | Gene Information cow ... PTI(404172) | Gene Information cow ... PTI(404172) |
| Quality Level 200 | Quality Level 300 | Quality Level 300 | Quality Level 300 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form saline solution |
| solubility H2O: 5 mg/mL | solubility H2O: >10 mg/mL | solubility H2O: ≥5 mg/mL | solubility H2O: soluble >10 mg/mL |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Protocols
Objective: To standardize a procedure for the enzymatic assay of Aprotinin.
Articles
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| A3428-250MG | 04061833360897 |
| A3428-100MG | 04061833360873 |
| A3428-10MG | 04061833360880 |
| A3428-25MG | 04061833360903 |
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What is the shelf life and where should I store the product after I have reconstitued it with H2O?
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