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A3547

Achromopeptidase from bacteria

lyophilized powder, ≥1,000 units/mg solid

Synonym(s):

ACP, Lysyl endopeptidase

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100000 units
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$197.00
500000 units
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$804.00
1000000 units
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$1,350.00

About This Item

NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
≥1,000 units/mg solid
Biological source:
bacterial (LYSOBACTER SP. BACTERIA M497-1)

$197.00

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biological source

bacterial (LYSOBACTER SP. BACTERIA M497-1)

Quality Level

200

form

lyophilized powder

specific activity

≥1,000 units/mg solid

mol wt

27 kDa

technique(s)

cell based assay: suitable

suitability

suitable for cell lysis

application(s)

diagnostic assay manufacturing

shipped in

dry ice

storage temp.

−20°C

Application

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections[1].

Biochem/physiol Actions

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C.
Achromopeptidase is a lysyl endopeptidase with a MW of ~27 kDa. It is useful for lysis of Gram-positive bacteria that are resistant to lysozyme.
pH Optimum for activity: pH 8.5 - 9
Approximately 500-1,500 un/ml achromopetidase can be used to lyse cells at a density of OD600=0.6 over 2 hours at 37 °C.

Physical form

crude powder

Other Notes

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

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biological source

bacterial (LYSOBACTER SP. BACTERIA M497-1)

biological source

-

biological source

-

biological source

bacterial (Staphylococcus sp.)

technique(s)

cell based assay: suitable

technique(s)

-

technique(s)

-

technique(s)

-

specific activity

≥1,000 units/mg solid

specific activity

≥20,000 units/mg solid

specific activity

2,000-5,000 units/mg protein

specific activity

≥500 units/mg protein

suitability

suitable for cell lysis

suitability

-

suitability

-

suitability

suitable for microbiological tests

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

form

lyophilized powder

form

partially purified powder

form

lyophilized powder

form

lyophilized powder

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000506234
0000394744
0000335811
0000394744
0000335811

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Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

Disposable platform for bacterial lysis and nucleic acid amplification based on a single USB-powered printed circuit board.
Shah, et al.
PLoS ONE, 18, e0284424-e0284424 (2023)
Georgios Chondrogiannis et al.
Scientific reports, 11(1), 6140-6140 (2021-03-19)
Enzymes are the cornerstone of modern biotechnology. Achromopeptidase (ACP) is a well-known enzyme that hydrolyzes a number of proteins, notably proteins on the surface of Gram-positive bacteria. It is therefore used for sample preparation in nucleic acid tests. However, ACP
Chelsey A Smith et al.
Lab on a chip, 23(3), 451-465 (2022-12-24)
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Global Trade Item Number

SKUGTIN
A3547-1MU04061833361085
A3547-100KU04061833361054
A3547-500KU04061832699547

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