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A3547

Achromopeptidase from bacteria

lyophilized powder, ≥1,000 units/mg solid

Synonyme(s) :

ACP, Lysyl endopeptidase

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100000 units
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A propos de cet article

NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
≥1,000 units/mg solid
Biological source:
bacterial (LYSOBACTER SP. BACTERIA M497-1)

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biological source

bacterial (LYSOBACTER SP. BACTERIA M497-1)

Quality Segment

200

form

lyophilized powder

specific activity

≥1,000 units/mg solid

mol wt

27 kDa

technique(s)

cell based assay: suitable

input

bacteria and yeast sample(s)

suitability

suitable for cell lysis

application(s)

diagnostic assay manufacturing

shipped in

dry ice

storage temp.

−20°C

Application

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections[1].

Biochem/physiol Actions

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C.
Achromopeptidase is a lysyl endopeptidase with a MW of ~27 kDa. It is useful for lysis of Gram-positive bacteria that are resistant to lysozyme.
pH Optimum for activity: pH 8.5 - 9
Approximately 500-1,500 un/ml achromopetidase can be used to lyse cells at a density of OD600=0.6 over 2 hours at 37 °C.

Physical form

crude powder

Other Notes

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

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Achromopeptidase from bacteria lyophilized powder, ≥1,000 units/mg solid

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description

lyophilized powder, ≥1,000 units/mg solid

description

partially purified powder, ≥20,000 units/mg solid

description

Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, 2,000-5,000 units/mg protein

description

Suitable for manufacturing of diagnostic kits and reagents., lyophilized powder

technique(s)

cell based assay: suitable

technique(s)

-

technique(s)

-

technique(s)

-

specific activity

≥1,000 units/mg solid

specific activity

≥20,000 units/mg solid

specific activity

2,000-5,000 units/mg protein

specific activity

≥500 units/mg protein

biological source

bacterial (LYSOBACTER SP. BACTERIA M497-1)

biological source

-

biological source

-

biological source

bacterial (Staphylococcus sp.)

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

form

lyophilized powder

form

partially purified powder

form

lyophilized powder

form

lyophilized powder

suitability

suitable for cell lysis

suitability

-

suitability

-

suitability

suitable for microbiological tests

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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