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A3812

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

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Size/SKUAvailabilityPrice
0.5 mL
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$249.00
1 mL
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$379.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162782
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d), IHC (p), WB
Citations:
48

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biological source

goat

Quality Segment

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

rabbit

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Anti-rabbit IgG (whole molecule) is produced in goat using purified rabbit IgG as the immunogen. Antibody is isolated from anti-rabbit IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins that do not specifically bind to rabbit IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations. Anti-Rabbit IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde.
Specificity of the antiserum is determined by immunoelectrophoresis prior to conjugation, versus normal rabbit serum and rabbit IgG. Identity and purity of the antibody is established by immunoelectrophoresis prior to conjugation. Electrophoresis of the product followed by diffusion versus anti-goat IgG and anti-goat whole serum results in single arcs of precipitation.
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It consists of a gamma (γ) heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity (Fab fragments). The third fragment does not possess antigen-binding activity and is known as fragment crystallizable (Fc).

Immunogen

Purified rabbit IgG

Application

Citrullination of antithrombin by PADI4 was analyzed by ELISA using alkaline phosphatase conjugated goat anti-rabbit IgG as the secondary diluted at 1:5000 in 0.05M carbonate/bicarbonate buffer (Ph 9.6).
Goat anti-rabbit IgG (whole molecule)-alkaline phosphatase antibody can be used for western blot and ELISA
It can also be used for immunohistochemistry (1:50) and dot bot (1:30,000) assays.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Preparation Note

Adsorbed to reduce background staining with human samples.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A3812

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

A7650

Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A4062

Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A4187

Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

description

affinity isolated antibody, buffered aqueous glycerol solution

description

affinity isolated antibody, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous glycerol solution

description

affinity isolated antibody, buffered aqueous glycerol solution

biological source

goat

biological source

rabbit

biological source

rabbit

biological source

rabbit

technique(s)

dot blot: 1:30,000, western blot: 1:30,000, direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50

technique(s)

direct ELISA: 1:7,000

technique(s)

direct ELISA: 1:30,000, western blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50

technique(s)

direct ELISA: 1:30,000, western blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

species reactivity

rabbit

species reactivity

-

species reactivity

goat

species reactivity

goat

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

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Storage Class

10 - Combustible liquids

WGK

WGK 2

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