Key Documents

Anti-Human Kappa Light Chains (Bound and Free)−Alkaline Phosphatase antibody produced in goat

Name: Anti-Human Kappa Light Chains (Bound and Free)−Alkaline Phosphatase antibody produced in goat
Brand: SIGMA

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Goat Anti-Human Kappa Light Chains (Bound and Free)−AP

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162518

Conjugate:

alkaline phosphatase conjugate

Clone:

polyclonal

Application:

DB, ELISA (d), IHC (p)

Citations:

Pricing and availability is not currently available.

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Properties

biological source

goat

Quality Segment

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... IGK@(50802)

Description

General description

Human κ light chains are antibody fragments that regulate immunological responses in cells. Increased expression of κ light chains has been linked to chronic lymphocytic leukemia . The variable fragment of the κ light chain gene has also been associated with vaccine-induced antibody response to the polysaccharide coat in Haemophilus influenzae type b . Anti-Human κ Light Chains (Bound and Free-Alkaline Phosphatase antibody is specific for human κ light chain when tested against human IgA, IgG, IgM, IgGλ, IgAλ, IgMλ, Bence Jones κ and λ myeloma proteins.

Immunogen

Purified Bence Jones κ light chain

Application

Anti-Human κ Light Chains (Bound and Free)-Alkaline Phosphatase antibody is suitable for use in ELISA. The product can also be used for dot blot (1:30,000) and immunohistochemistry (1:50 using formalin-fixed, paraffin-embedded sections).

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Mouse-Human chimeric IgG′s specific for Pseudomonas aeruginosa serogroup 06 lipopolysaccharide were identified by ELISA in transfected CHO cell supernatants using Alkaline phosphatase conjugated goat anti-Human Kappa Light chains.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A7164	A2904	A3187
Sigma-Aldrich A3813 Anti-Human Kappa Light Chains (Bound and Free)−Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A7164 Anti-Human Kappa Light Chains (Bound and Free)−Peroxidase antibody produced in goat	Sigma-Aldrich A2904 Anti-Human Lambda Light Chains (Bound and Free)−Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A3187 Anti-Human IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat
description affinity isolated antibody, buffered aqueous glycerol solution	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous glycerol solution	description affinity isolated antibody, buffered aqueous glycerol solution
technique(s) direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, dot blot: 1:30,000	technique(s) direct ELISA: 1:1,000	technique(s) direct ELISA: 1:2,000-1:21,000	technique(s) direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000
biological source goat	biological source goat	biological source goat	biological source goat
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
conjugate alkaline phosphatase conjugate	conjugate peroxidase conjugate	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate
species reactivity human	species reactivity -	species reactivity human	species reactivity human
shipped in wet ice	shipped in dry ice	shipped in wet ice	shipped in wet ice

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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