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Bovine Serum Albumin

Name: Bovine Serum Albumin
Brand: SIGMA
Price: 276 USD
Availability: InStock

heat shock fraction, pH 5.2, ≥96%

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

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Size/SKU	Availability	Price
50 g	Available to ship TODAYfromMILWAUKEE	$276.00
100 g	Available to ship TODAYfromMILWAUKEE	$400.00
500 g	Available to ship TODAYfromMILWAUKEE	$1,500.00
1 kg	Check Cart for Availability	$2,160.00
5 kg	Check Cart for Availability	Inquire

About This Item

CAS Number:

9048-46-8

UNSPSC Code:

12352202

EC Number:

232-936-2

NACRES:

NA.27

MDL number:

MFCD00130384

eCl@ss:

32160409

Biological source:

bovine

Assay:

≥96%

Form:

lyophilized powder

Technique(s):

blood typing: suitable, microbiological culture: suitable

Impurities:

BT and VSV virus, tested

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Properties

biological source

bovine

Quality Segment

200

assay

≥96%

form

lyophilized powder

purified by

heat shock fractionation

packaging

poly bottle of

origin

USA origin

technique(s)

blood typing: suitable, microbiological culture: suitable

impurities

BT and VSV virus, tested

loss

≤5%

pH

5.2

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

foreign activity

BT Virus, none detected, VSV Virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Description

General description

Bovine serum albumin (BSA) is a 66.4 kDa, water-soluble protein composed of 583 amino acids. It has a single polypeptide chain with three homologous domains formed by six α-helices. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating. BSA has been used as a model protein in various applications, including immunodiagnostic procedures, cell culture media and clinical chemistry. Serum albumins facilitates drug disposition and efficacy by maintaining osmotic blood pressure.

Application

Bovine Serum Albumin (BSA) has been used:

to study its adsorption on a planar poly(acrylic acid) (PAA) brush layer through fixed-angle optical reflectometry
to measure its adsorption on a variety of thin films by means of total internal reflection spectroscopy
as a constituent of Krebs-Ringer bicarbonate buffer, which acts as a perfusate during placental perfusion

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Analysis Note

Remainder mostly globulins.

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This Item	A3059	A4919	A4503
$Bovine Serum Albumin heat shock fraction, pH 5.2, ≥96%$ Sigma-Aldrich A3912 Bovine Serum Albumin	$Bovine Serum Albumin heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%$ Sigma-Aldrich A3059 Bovine Serum Albumin	$Bovine Serum Albumin heat shock fraction, protease free, low endotoxin, suitable for cell culture, pH 7, ≥98%$ Sigma-Aldrich A4919 Bovine Serum Albumin	$Bovine Serum Albumin cold ethanol fraction, pH 5.2, ≥96%$ Sigma-Aldrich A4503 Bovine Serum Albumin
description heat shock fraction, pH 5.2, ≥96%	description heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%	description heat shock fraction, protease free, low endotoxin, suitable for cell culture, pH 7, ≥98%	description cold ethanol fraction, pH 5.2, ≥96%
assay ≥96%	assay ≥98%	assay ≥98%	assay ≥96%
biological source bovine	biological source bovine	biological source bovine	biological source bovine
origin USA origin	origin USA origin	origin USA origin	origin USA origin
technique(s) blood typing: suitable, microbiological culture: suitable	technique(s) ELISA: suitable, western blot: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) blood typing: suitable, microbiological culture: suitable, ELISA: suitable
Quality Level 200	Quality Level 300	Quality Level 200	Quality Level 300
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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