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Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

Name: Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit
Brand: SIGMA
Price: 265 USD
Availability: InStock

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Rabbit Anti-Goat IgG (whole molecule)–AP

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Size/SKU	Availability	Price
0.5 mL	Available to ship TODAYfromMILWAUKEE	$265.00
1 mL	Available to ship TODAYfromMILWAUKEE	$473.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162335

Conjugate:

alkaline phosphatase conjugate

Clone:

polyclonal

Application:

ELISA (d), IHC (p), WB

Citations:

$265.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

goat

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Description

General description

Immunoglobulins (Igs) belongs immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobin have two heavy (H) and two light (L), held together by disulphide linkages. Each light comprises of one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region.

Immunogen

purified goat IgG

Application

Alkaline phosphatase conjugated rabbit anti goat IgG was used as the secondary for ELISA assays to detect the binding of bacterial proteins to C components. The antibody was used at 1:5000 dilutions and detected using P-nitrophenyl phosphate at 1mg/ml (Sigma) as the substrate.

Anti-Goat IgG (whole molecule)-alkaline phosphatase antibody can be used in western blotting and immunoblotting (diluted 1: 20,000) .

Biochem/physiol Actions

IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus, they play a crucial role in facilitating cytological immune responses. Anti-goat IgG (whole molecule)-alkaline phosphatase antibody (diluted 1: 5000) can be used for anti-OVA IgG detection. Rabbit anti- goat IgG (whole molecule)-alkaline phosphatase antibody reacts specifically with all goat immunoglobulins.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 10 mM glycine, 1 mM MgCl₂, 50% glycerol and 15 mM sodium azide

Preparation Note

Adsorbed to reduce background staining with human samples.

Other Notes

Antibody adsorbed with human serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A4187	A3562	A3688
Sigma-Aldrich A4062 Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit	Sigma-Aldrich A4187 Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit	Sigma-Aldrich A3562 Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich A3688 Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat
description affinity isolated antibody, buffered aqueous glycerol solution	description affinity isolated antibody, buffered aqueous glycerol solution	description affinity isolated antibody, buffered aqueous glycerol solution	description affinity isolated antibody, buffered aqueous glycerol solution
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
biological source rabbit	biological source rabbit	biological source goat	biological source goat
conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate
species reactivity goat	species reactivity goat	species reactivity mouse	species reactivity mouse
technique(s) western blot: 1:30,000, direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000, direct ELISA: 1:30,000	technique(s) dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000, direct ELISA: 1:30,000	technique(s) western blot: 1:30,000, dot blot: 1:30,000, direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal

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Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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