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A4174

Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Rabbit Anti-Goat IgG (whole molecule)–HRP

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1 mL
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$456.00
$387.60

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162340
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA (d)
Citations:
39

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biological source

rabbit

Quality Level

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

goat

should not react with

human

technique(s)

direct ELISA: 1:10,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus they play a crucial role in facilitating cytological immune responses. Anti-goat IgG (whole molecule)–peroxidase antibody can be used as a secondary antibody in ovarian tissue microarray. Rabbit anti-goat IgG (whole molecule)-peroxidase antibody reacts specifically with all goat IgG but shows no reactivity with human serum proteins.

Immunogen

Purified goat IgG.

Application

Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in rabbit is suitable for use in immunoblot and immunohistochemistry. The product can also be used for direct ELISA (1:10,000).
The presence of pleiotrophin in HUVEC cell culture medium was analyzed by western blot using HRP-conjugated rabbit anti-goat IgGas the secondary at a dilution of 1:7500 in TBST.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Other Notes

Antibody adsorbed with human serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
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Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

A4174

Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit

Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

A8919

Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit

Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

A5420

Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat IgG fraction of antiserum, buffered aqueous solution

Sigma-Aldrich

A8275

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat

technique(s)

direct ELISA: 1:10,000

technique(s)

direct ELISA: 1:30,000, dot blot: 1:2,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, western blot (chemiluminescent): 1:160,000

technique(s)

direct ELISA: 1:40,000, dot blot: 1:80,000-1:160,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200

technique(s)

direct ELISA: 1:10,000

species reactivity

goat

species reactivity

-

species reactivity

goat

species reactivity

-

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

goat

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

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Certificates of Analysis (COA)

Lot/Batch Number
0000488115
0000488115
0000488113
0000488113
0000473174

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Rebekka Mauser et al.
Epigenetics & chromatin, 10(1), 45-45 (2017-09-28)
Histone post-translational modifications (PTMs) play central roles in chromatin-templated processes. Combinations of two or more histone PTMs form unique interfaces for readout and recruitment of chromatin interacting complexes, but the genome-wide mapping of coexisting histone PTMs remains an experimentally difficult task.
Sanja Cabric et al.
Tissue engineering. Part A, 16(3), 961-970 (2009-12-22)
In pancreatic islet transplantation, early revascularization is necessary for long-term graft function. We have shown in in vitro and in vivo models that modification with surface-attached heparin protects the islets from acute attack by the innate immune system of the
Peter Horak et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 11(24 Pt 1), 8585-8591 (2005-12-20)
Epithelial ovarian cancer is the most common cause of mortality from gynecologic malignancies. Due to advanced stage at diagnosis, most patients need systemic treatment in addition to surgery. Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the
View All Related Papers

Global Trade Item Number

SKUGTIN
A4174-.5ML04061835805051
A4174-1ML04061837514296

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