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Monoclonal Anti-Myosin (Skeletal, Fast)−Alkaline Phosphatase antibody produced in mouse

Name: Monoclonal Anti-Myosin (Skeletal, Fast)−Alkaline Phosphatase antibody produced in mouse
Brand: SIGMA

clone MY-32, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Myosin (Skeletal, Fast) antibody produced in mouse

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00145920

Conjugate:

alkaline phosphatase conjugate

Clone:

MY-32, monoclonal

Application:

IF (d)

Citations:

Pricing and availability is not currently available.

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Properties

biological source

mouse

Quality Segment

200

conjugate

alkaline phosphatase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MY-32, monoclonal

form

buffered aqueous glycerol solution

species reactivity

rat, chicken, rabbit, mouse, human, bovine, guinea pig, feline

technique(s)

direct immunofluorescence: 1:150 using formalin-fixed, paraffin-embedded human or animal skeletal muscle sections

isotype

IgG1

UniProt accession no.

P12882

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... MYH1(4619), MYH2(4620)
mouse ... Myh1(17879), Myh2(17882)
rat ... Myh1(287408), Myh2(691644)

Description

General description

Localizes an epitope on the myosin heavy chain. Stains the fast (type II) and neonatal isomyosin molecules found in skeletal muscle, but does not stain cardiac muscle, smooth muscle or non-muscle myosin in cultured cells. Does react with human rhabdomyosarcomas.

Monoclonal Anti-Myosin (Skeletal, Fast) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

rabbit muscle myosin.

Application

Monoclonal Anti-Myosin (Skeletal, Fast) Alkaline Phosphatase antibody produced in mouse has been used for the detection and localization of skeletal muscle fast and neonatal myosins using immunohistochemistry. It may be used in muscle fiber typing, in studies of in vivo and in vitro muscle development and in the diagnosis of rhabdomyosarcomas.

The level of mysosin (fast) in serum samples from sportsmen with past injury was determined by western blot using monoclonal mouse anti-myosin (skeletal/fast) as the primary antibody at a dilution of 1:90000.

Biochem/physiol Actions

Monoclonal Anti-Myosin (Skeletal, Fast) specifically binds to an epitope in adult rat skeletal myosin heavy chains IIa, IIb and IId (IIx) (in fast-twitch fibers)†. The antibody product is also reactive in humans, bovines, cats, rabbits, mice, guinea pigs and chickens.

Myosin (Skeletal, Fast) is useful not only in fiber typing but also in detection of myogenic tumors. It enables retrospective as well as prospective fiber typing using formalin-fixed, paraffin-embedded material.

Myosins are motor proteins that interact with actin filaments to regulate cell movement. Myosins also modulate cell shape and signaling. Skeletal muscle myosins have three ′fast′ myosin heavy chains isoforms (IIa, IIx, and IIb), a ′slow′ beta-myosin heavy chain isoform and three major myosin light-chain (MLC) isoforms. These myosin isoforms regulate skeletal muscle shortening.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂, 50% glycerol, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4200670	M8421	M7786
Sigma-Aldrich A4335 Monoclonal Anti-Myosin (Skeletal, Fast)−Alkaline Phosphatase antibody produced in mouse	Sigma-Aldrich SAB4200670 Anti-Myosin (Skeletal, Slow) antibody, Mouse monoclonal	Sigma-Aldrich M8421 Monoclonal Anti-Myosin (Skeletal, Slow) antibody produced in mouse	Sigma-Aldrich M7786 Monoclonal Anti-Myosin (Smooth) antibody produced in mouse
description clone MY-32, purified from hybridoma cell culture	description clone NOQ7.5.4D, purified from hybridoma cell culture	description clone NOQ7.5.4D, ascites fluid	description clone hSM-V, ascites fluid
antibody form purified immunoglobulin	antibody form purified from hybridoma cell culture	antibody form ascites fluid	antibody form ascites fluid
conjugate alkaline phosphatase conjugate	conjugate -	conjugate unconjugated	conjugate unconjugated
Gene Information human ... MYH1(4619), MYH2(4620) mouse ... Myh1(17879), Myh2(17882) rat ... Myh1(287408), Myh2(691644)	Gene Information human ... MYH7(4625)	Gene Information human ... MYH7(4625) mouse ... Myh7(140781) rat ... Myh7(29557)	Gene Information human ... MYH11(4629)
clone MY-32, monoclonal	clone NOQ7.5.4D, monoclonal	clone NOQ7.5.4D, monoclonal	clone hSM-V, monoclonal
species reactivity rat, chicken, rabbit, mouse, human, bovine, guinea pig, feline	species reactivity sheep, goat, chicken, feline, guinea pig, pig, rabbit, hamster, canine, rat, human, mouse, bovine	species reactivity sheep, rat, bovine, hamster, pig, canine, feline, goat, chicken, mouse, rabbit, human, guinea pig	species reactivity guinea pig, human, pig, canine, rabbit, chicken
biological source mouse	biological source mouse	biological source mouse	biological source mouse

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10 - Combustible liquids

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