A4789
Anti-Mouse IgA (α-chain specific)−Peroxidase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Goat Anti-Mouse IgA (α-chain specific)−HRP
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About This Item
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA (d)
Citations:
28
Pricing and availability is not currently available.
biological source
goat
Quality Level
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:10,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
IgA antibody plays an essential role in mucosal immunity and binds with immunogens/pathogens to restrict them from entering the mucosal membrane. Anti-mouse IgA (α-chain specific)-peroxidase antibody can be used for determining the secretory IgA (sIgA) in intestinal fluid. It can also be used in direct ELISA. Goat anti-mouse IgA (α-chain specific)-peroxidase antibody reacts specifically with mouse IgA.
Immunogen
Purified mouse IgA
Application
Anti-mouse IgA (α-chain specific)-peroxidase antibody can be used in modified indirect DIBA (dot- immunobinding assay) .
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)
ELISAS were performed using HRP conjugated gaot anti-mouse IgA as the secondary antibody at a dilution of 1:10000 for a 1 hour incubation at 37 degrees to detect IgA levels in mouse fecal extracts.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.
Preparation Note
Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p 215 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) direct ELISA: 1:10,000 | technique(s) direct ELISA: 1:15,000 | technique(s) direct ELISA: 1:10,000 | technique(s) direct ELISA: 1:30,000, western blot: 1:30,000 |
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody |
| biological source goat | biological source goat | biological source goat | biological source goat |
| conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate alkaline phosphatase conjugate |
| form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous glycerol solution |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in wet ice |
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Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
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Related Content
A C Rodrigues et al.
Journal of applied microbiology, 89(3), 404-414 (2000-10-06)
The effect of Saccharomyces boulardii on the immune system was evaluated, comparing germ-free Swiss/NIH mice monoassociated with the probiotic with germ-free mice. Saccharomyces boulardii colonized the gut of germ-free mice and survived the gastrointestinal conditions. An increase in sIgA production
Hao Feng et al.
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Sidonia Fagarasan et al.
Nature reviews. Immunology, 3(1), 63-72 (2003-01-04)
Immunoglobulin A is the most abundant immunoglobulin isotype in mucosal secretions. In this review, we summarize recent advances in our understanding of the sites, mechanisms and functions of intestinal IgA synthesis in mice. On the basis of these recent findings
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A4789-.25ML | 04061835805181 |
| A4789-1ML | 04061833375013 |
| A4789-.5ML | 04061835805198 |