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A5187

Anti-Sheep IgG (whole molecule)–Alkaline Phosphatase antibody produced in donkey

affinity isolated antibody, buffered aqueous glycerol solution

別名:

Donkey Anti-Sheep IgG (whole molecule)–AP

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あなたへ/カタログ番号在庫状況単価
0.5 mL

在庫あり出荷可能fromŽsìŽs‘å’¬368

￥35,000
1 mL
カートの在庫状況を確認する
￥105,000

この商品について

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162844
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
ELISA (d), WB
Citations:
10

￥35,000

在庫あり出荷可能詳細

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biological source

donkey

Quality Segment

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

technique(s)

direct ELISA: 1:30,000, western blot: 1:30,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Affinity isolated antibody is obtained from anti-sheep IgG antiserum by immunospecific purification which removes essentially all donkey serum proteins, including immunoglobulins, which do not specifically bind to sheep IgG. Anti-Sheep IgG is conjugated to alkaline phosphatase by protein cross-linking with 0.2% glutaraldehyde. Specificity of the antiserum is determined by immunoelectrophoresis (IEP), prior to conjugation. By IEP, the antiserum reacts specifically with normal sheep serum and sheep IgG. Identity and purity of the antibody is established by immunoelectrophoresis, prior to conjugation. Electrophoresis of the product followed by diffusion against anti-horse IgG and anti-donkey whole serum results in single arcs of precipitation.
IgGs are glycoprotein antibodies that modulate several immune responses. Sheep IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-sheep IgGs are useful tools for the analysis of target proteins. Anti-Sheep IgG (whole molecule)-Alkaline Phosphatase antibody binds to all sheep immunoglobulins.

Immunogen

Purified sheep IgG

Application

Anti-Sheep IgG (whole molecule)-Alkaline Phosphatase antibody is suitable for use in ELISA .
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Solid-Phase Decapeptide Assays were performed using alkaline phosphatase conjugated donkey anti-sheep IgG at 1:1000 for 2-3 hours at room temperature.
The anibody may be used in applications such as immunoblotting, dot blot and direct ELISA at a dilution of 1:30,000

Biochem/physiol Actions

normal sheep serum and sheep IgG

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1 mM MgCl2, 50% glycerol, 1% bovine serum albumin, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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当該品目
A3415A3687A4187
Anti-Sheep IgG (whole molecule)–Alkaline Phosphatase antibody produced in donkey affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A5187

Anti-Sheep IgG (whole molecule)–Alkaline Phosphatase antibody produced in donkey

抗-ヒツジ IgG（全分子）–ペルオキシダーゼ ロバ宿主抗体 affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

A3415

抗-ヒツジ IgG（全分子）–ペルオキシダーゼ ロバ宿主抗体

抗ウサギIgG (全分子)-アルカリフォスファターゼ ヤギ宿主抗体 affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A3687

抗ウサギIgG (全分子)-アルカリフォスファターゼ ヤギ宿主抗体

抗ヤギIgG (全分子)-アルカリフォスファターゼ ウサギ宿主抗体 affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A4187

抗ヤギIgG (全分子)-アルカリフォスファターゼ ウサギ宿主抗体

description

affinity isolated antibody, buffered aqueous glycerol solution

description

affinity isolated antibody, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous glycerol solution

description

affinity isolated antibody, buffered aqueous glycerol solution

technique(s)

direct ELISA: 1:30,000, western blot: 1:30,000

technique(s)

direct ELISA: 1:10,000

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

biological source

donkey

biological source

donkey

biological source

goat

biological source

rabbit

conjugate

alkaline phosphatase conjugate

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

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保管分類

10 - Combustible liquids

wgk

WGK 1

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