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Anti-Mouse IgG (whole molecule)−Agarose antibody produced in goat

Name: Anti-Mouse IgG (whole molecule)−Agarose antibody produced in goat
Brand: SIGMA
Price: 280 USD
Availability: InStock

affinity isolated antibody, suspension

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Size/SKU	Availability	Price
2 mL	Check Cart for Availability	$280.00
5 mL	Check Cart for Availability	$515.00
25 mL	Check Cart for Availability	$2,070.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD03791275

Conjugate:

agarose conjugate

Clone:

polyclonal

Application:

IP, ODD

Citations:

$280.00

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Properties

biological source

goat

Quality Segment

200

conjugate

agarose conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

suspension

species reactivity

mouse

extent of labeling

≥5 mg per mL

technique(s)

Ouchterlony double diffusion: suitable, immunoprecipitation (IP): suitable

matrix

Cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix spacer

1 atom

capacity

0.4 mg/mL, resin binding capacity (mouse IgG)

storage temp.

2-8°C

target post-translational modification

unmodified

Description

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mice consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE.

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Goat Anti-Mouse IgG (whole molecule)-Agarose antibodies bind to all mouse IgG subclasses.

Immunogen

IgG isolated from pooled normal mouse serum

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunoprecipitation (1 paper)

Goat Anti-Mouse IgG (whole molecule)-Agarose antibodies have been used for immunoprecipiation assays. The product can also be used for Ouchterlony double diffusion applications.

Soluble antigens from meningococci extracts were mixed with ascitic fluid containing primary antibody and 50 μl of agarose-linked goat anti-mouse IgG for 5 hours at 4 °C.

Biochem/physiol Actions

IgG helps in opsonization, complement fixation and antibody-dependent cell-mediated cytotoxicity. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. IgG2a of IgG has cytotropic properties and IgG2 molecules have the ability to activate complement.

Physical form

Suspension in 0.5 M NaCl containing preservative

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A8914	A3543	A5795
Sigma-Aldrich A6531 Anti-Mouse IgG (whole molecule)−Agarose antibody produced in goat	Sigma-Aldrich A8914 Anti-Rabbit IgG (whole molecule)−Agarose antibody produced in goat	$Anti-Human IgG (whole molecule)−Agarose antibody produced in goat IgG fraction of antiserum, PBS suspension$ Sigma-Aldrich A3543 Anti-Human IgG (whole molecule)−Agarose antibody produced in goat	Sigma-Aldrich A5795 Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in rabbit
description affinity isolated antibody, suspension	description affinity isolated antibody, saline suspension	description IgG fraction of antiserum, PBS suspension	description affinity isolated antibody, buffered aqueous solution
conjugate agarose conjugate	conjugate agarose conjugate	conjugate agarose conjugate	conjugate peroxidase conjugate
biological source goat	biological source goat	biological source goat	biological source rabbit
technique(s) Ouchterlony double diffusion: suitable, immunoprecipitation (IP): suitable	technique(s) immunoelectrophoresis: suitable	technique(s) immunoelectrophoresis: suitable	technique(s) direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:750, western blot (chemiluminescent): 1:80,000-1:160,000 using rat PC12 cell extract
species reactivity mouse	species reactivity -	species reactivity human	species reactivity rat
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody
form suspension	form saline suspension	form PBS suspension	form buffered aqueous solution

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Storage Class

10 - Combustible liquids

Pictograms

GHS07

Signal Word

Warning

Hazard Codes

H317

Precautionary Statements

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

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