This antibody is NOT tested for western blot application. There are references that site using this antibody at dilutions of 1:200- 1:500.
A6667
Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment−Peroxidase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|---|---|
1 mL | Check Cart for Availability | $340.00 |
About This Item
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA (d)
Citations:
80
biological source
goat
Quality Segment
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:10,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus they play a crucial role in facilitating cytological immune responses. Anti-rabbit IgG (whole molecule), F(ab′)2 fragment-peroxidase antibody can be used to detect immune-complexes. The product can also be used in direct ELISA. Goat anti- rabbit IgG (whole molecule), F(ab′)2 fragment-peroxidase antibody reacts specifically with all rabbit immunoglobulins.
Immunogen
Purified rabbit IgG.
Application
Anti-rabbit IgG (whole molecule), F(ab′)2 fragment-peroxidase antibody can be used in immunoprecipitation and western blotting.
Proteins from lysed Leishmania amazonensis cells were analyzed by western blot using HRP-conjugated goat anti-rabbit Fc specific IgG as the secondary antibody.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.
Preparation Note
Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| description affinity isolated antibody, buffered aqueous solution | description affinity isolated antibody, buffered aqueous solution | description IgG fraction of antiserum, buffered aqueous solution | description affinity isolated antibody, buffered aqueous solution |
| technique(s) direct ELISA: 1:10,000 | technique(s) direct ELISA: 1:10,000 | technique(s) direct ELISA: 1:40,000, western blot (chemiluminescent): 1:5,000 using detecting rat primary antibody, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 | technique(s) direct ELISA: 1:10,000 |
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form IgG fraction of antiserum | antibody form affinity isolated antibody |
| biological source goat | biological source goat | biological source rabbit | biological source goat |
| conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate peroxidase conjugate |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
Still not finding the right product?
Try our Product Selector Tool to narrow your options
Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
WGK
WGK 3
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
Hi, my lab recently purchased this product. I just noticed that the specification doesn't show the recommended concentration for immunoblots. As we purchased it for doing westerns, we were wondering if this product is at all compatible. Thank you.
1 answer-
Helpful?
-