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Anti-Human IgM (μ-chain specific)−Peroxidase antibody produced in goat

Name: Anti-Human IgM (μ-chain specific)−Peroxidase antibody produced in goat
Brand: SIGMA
Price: 4176.76 ZAR

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Human IgM (μ-chain specific)−HRP

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Size/SKU	Availability	Price
1 mL	Please contact Customer Service for Availability	R 4 262,00 R 4 176,76

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162464

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

ELISA (d)

Citations:

R 4 176,76

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Properties

biological source

goat

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

concentration

0.5-4 mg/mL

technique(s)

direct ELISA: 1:10,000

isotype

IgM

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Human IgM antibodies are glycoproteins that are mainly produced by the B-1 cells and regulate the physiology and growth of the human B cell reserve. Circulating monoclonal IgM antibody that binds to myelin-associated glycoprotein has been linked to the demyelination of peripheral nerves in humans . Anti-human IgM μ-chain specific−peroxidase antibody is specific for human IgM when tested against human IgA, IgG, IgM, Bence Jones κ and Lambda myeloma proteins.

There are five immunoglobulin classes in humans. Out of these, immunoglobulin M (IgM) is a high molecular weight protein that has five to six subunits. IgM monomers are made of two heavy chains and two light chains connected by a disulfide bond. Human serum shows low concentration of IgM monomers. It has a high carbohydrate content of about 12%. It is the first immunoglobulin produced by neonates.

Immunogen

human IgM

Application

Anti-Human IgM (μ-chain specific)-Peroxidase antibody has been used in direct binding assays to detect antiphospholipid antibodies (aPL).

Anti-human IgM (μ-chain specific)-Peroxidase antibody is suitable for use in direct ELISA (1:10,000 and 1:5000). It has also been used in indirect ELISA (1:2000). The concentration of IgM in supernatant samples was determined by ELISA using HRP-conjugated goat anti-human chain specific IgG as the secondary antibody. The antibody has also been used in Western blotting.

Biochem/physiol Actions

Immunoglobulin M (IgM) acts as an antigen specific part of the B cell antigen receptor on the surface of B lymphocytes that are not stimulated, in its monomeric form. Polymeric IgM molecules also serve as important activators of the classical complement cascade. IgM is essential in agglutination and cytolytic reactions.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% BSA with preservative.

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A0420	A6029	A3673
Sigma-Aldrich A6907 Anti-Human IgM (μ-chain specific)−Peroxidase antibody produced in goat	Sigma-Aldrich A0420 Anti-Human IgM (μ-chain specific)−Peroxidase antibody produced in goat	Sigma-Aldrich A6029 Anti-Human IgG (γ-chain specific)−Peroxidase antibody produced in goat	Sigma-Aldrich A3673 Anti-Mouse IgG (γ-chain specific)−Peroxidase antibody produced in goat
conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate
technique(s) direct ELISA: 1:10,000	technique(s) direct ELISA: 1:50,000, dot blot: 1:100,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200	technique(s) direct ELISA: 1:10,000	technique(s) direct ELISA: 1:15,000
species reactivity human	species reactivity human	species reactivity human	species reactivity mouse
biological source goat	biological source goat	biological source goat	biological source goat
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
isotype IgM	isotype -	isotype -	isotype -

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pictograms

GHS07,GHS09

signalword

Warning

hcodes

H315,H317,H319,H411

pcodes

P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

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