A7345
Anti-V5 Agarose Affinity Gel antibody produced in mouse
purified immunoglobulin, clone V5-10
Synonym(s):
Monoclonal Anti-V5
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 mL | Check Cart for Availability | $751.00 |
About This Item
Conjugate:
agarose conjugate
Clone:
V5-10, monoclonal
Application:
IP, affinity chromatography
Citations:
161
$751.00
Check Cart for Availability
biological source
mouse
Quality Level
conjugate
agarose conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
V5-10, monoclonal
analyte chemical class(es)
proteins (V5)
technique(s)
affinity chromatography: suitable, immunoprecipitation (IP): suitable
capacity
2.5 nmol/mL, gel (V5 fusion protein)
shipped in
wet ice
storage temp.
2-8°C
General description
Anti-V5 Agarose Affinity Gel is made of purified immunoglobulin fraction of monoclonal anti-V5 isolated from ascites fluid of the V5-10 hybridoma coupled to cyanogen bromide-activated beaded agarose. The purified monoclonal antibody is immobilized on agarose.
The Anti-V5 Agarose Affinity Gel is composed of the monolonal anti-V5 antibody conjugated to cyanogen bromide-activated beaded agarose. The antibody recognizes the V5-tagged recombinant fusion proteins expressed in transfected mammalian cells.
Application
Biochem/physiol Actions
Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins
Recombinant DNA technology enables the attachment of genes of interest to specific sequences or genes that can provide ′affinity handles′ designed to enable the selective identification and purification of the protein of interest.[6] The addition of a V5 tag sequence (GKPIPNPLLGLDST)[6] to a given gene creates a stable fusion product that does not appear to interfere with the bioactivity of the protein, or with the biodistribution of the tagged product. Many recombinant proteins have been engineered to express a short sequence derived from the V5 from simian virus known as V5-tag.[7] Anti-“tag” agarose conjugates may facilitate the detection, isolation and purification of these proteins.[7]
Physical form
Suspension of beaded agarose (1:1 v/v) in 0.01 M phosphate buffered saline, containing 15 mM sodium azide.
Preparation Note
For continuous use and extended storage, store at 2 °C to 8 °C. Do not freeze.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified from hybridoma cell culture |
| biological source mouse | biological source - | biological source mouse | biological source mouse |
| clone V5-10, monoclonal | clone M2, monoclonal | clone V5-10, monoclonal | clone V5-10, monoclonal |
| conjugate agarose conjugate | conjugate agarose conjugate | conjugate CY3 conjugate | conjugate unconjugated |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in dry ice |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| A7345-1ML | 04061833384046 |