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A8076

Anti-Phosphoserine−Agarose antibody, Mouse monoclonal

clone PSR-45, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Phosphoserine, Phospho Ser, Phospho serine, Phospho−Ser, Phospho−serine

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0.5 mL
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$623.00

About This Item

NACRES:
NA.44
UNSPSC Code:
12352203
Conjugate:
agarose conjugate
Clone:
PSR-45, monoclonal
Application:
IP
Citations:
7

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biological source

mouse

Quality Level

200

conjugate

agarose conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

PSR-45, monoclonal

form

buffered aqueous solution

technique(s)

immunoprecipitation (IP): suitable

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Monoclonal Anti-Phosphoserine (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

phosphoserine conjugated to Keyhole Limpet Hemocyanin (KLH).

Application

Monoclonal Anti-Phosphoserine - Agarose may be used for the immunoprecipitation or immuno-affinity purification of serine phosphoproteins.The antibody may be used for the localization of some phosphoserine containing proteins using the immunoblotting method.

Biochem/physiol Actions

By ELISA and dot blot, the antibody reacts specifically with phosphorylated serine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphoserine-containing proteins. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody due to steric hindrance of the recognition site.
Reversible phosphorylation of proteins is an important post-translational modification that plays a regulatory role in the expression of most proteins in the cells. Reversible phosphorylation at multiple serine, tyrosine and threonine residues mediate numerous signalling pathways in both prokaryotic and Eukaryotic cells. Cellular proteins with phosphorylated serine increase many fold by the activation of serine kinases. Most mitogenic receptor systems such as EGF, PDGF, insulin receptors contain serine/threonine/tyrosine kinase domains that undergo autophosphorylation when receptors bind to the respective ligands. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases,3 and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr.
Monoclonal Anti-Phosphoserine may be used for the identification of proteins containing phosphorylated serine both as the free amino acid or when conjugated to carriers such as BSA or KLH. It does not react with non-phosphorylated serine, phosphorylated tyrosine or threonine, AMP or ATP.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
A1806P5747A5964
Anti-Phosphoserine−Agarose antibody, Mouse monoclonal clone PSR-45, purified from hybridoma cell culture

Sigma-Aldrich

A8076

Anti-Phosphoserine−Agarose antibody, Mouse monoclonal

Monoclonal Anti-Phosphotyrosine−Agarose antibody produced in mouse clone PT-66, purified immunoglobulin, PBS solution

Sigma-Aldrich

A1806

Monoclonal Anti-Phosphotyrosine−Agarose antibody produced in mouse

Anti-Phosphoserine antibody, Mouse Monoclonal clone PSR-45, purified from hybridoma cell culture

Sigma-Aldrich

P5747

Anti-Phosphoserine antibody, Mouse Monoclonal

Monoclonal Anti-Phosphotyrosine–Peroxidase antibody produced in mouse clone PT-66, purified immunoglobulin, lyophilized powder

Sigma-Aldrich

A5964

Monoclonal Anti-Phosphotyrosine–Peroxidase antibody produced in mouse

clone

PSR-45, monoclonal

clone

PT-66, monoclonal

clone

PSR-45, monoclonal

clone

PT-66, monoclonal

conjugate

agarose conjugate

conjugate

agarose conjugate

conjugate

unconjugated

conjugate

peroxidase conjugate

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

technique(s)

immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): suitable

technique(s)

dot blot: suitable, indirect ELISA: 0.3-0.6 μg/mL using phosphoserine conjugated to BSA, microarray: suitable, western blot: 2.5-5.0 μg/mL using total rat brain extract.

technique(s)

direct ELISA: 1:60,000 using Phosphotyrosine-BSA, dot blot: 1:40,000-1:200,000 using phosphotyrosine-BSA using chromogenic and chemiluminescent substrates, respectively

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000463764
0000463764
0000384969
0000384969
0000214421

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Global Trade Item Number

SKUGTIN
A8076-1ML04061838160904
A8076-.5ML04061838160898

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