This product is intended for research use only and has not been validated for lateral flow assays. It is the responsibility of the user to determine whether this product will be appropriate for the intended application.
A8549
Albumin, biotin labeled bovine
lyophilized powder
Synonym(s):
Biotin labeled albumin
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 mg | Please contact Customer Service for Availability | $195.00 |
About This Item
Biological source:
bovine
Assay:
≥80% protein basis (biuret)
Form:
lyophilized powder
Technique(s):
bioconjugation: suitable
Concentration:
≥97% protein in anhydrous DMSO (spectralphotometric; calc. on anhydrous substance), ≥98% (Assay of Albumin (Agarose gel electrophoresis))
biological source
bovine
Quality Level
assay
≥80% protein basis (biuret)
form
lyophilized powder
concentration
≥97% protein in anhydrous DMSO (spectralphotometric; calc. on anhydrous substance), ≥98% (Assay of Albumin (Agarose gel electrophoresis))
extent of labeling
8-16 mol biotin per mol albumin
technique(s)
bioconjugation: suitable
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
Application
Biotin-conjugated bovine serum albumin binds selectively to different vascular subsets in lymphoid tissues, as demonstrated by both fluorescent and enzymohistochemical techniques, and thus offers a histochemical probe for identification of select microvascular endothelia in lymphoid tissues.
Biotin-conjugated bovine serum albumin binds selectively to different vascular subsets in lymphoid tissues, as demonstrated by both fluorescent and enzymohistochemical techniques, and thus offers a histochemical probe for identification of select microvascular endothelia in lymphoid tissues. In spleen, biotin-BSA binds to red pulp sinuses, but not white pulp vessels (including the central arteriole) nor the marginal sinus. In the thymus, prominent binding was restricted to the capillaries of the corticomedullary area.
Biotin labeled bovine albumin has been used as a component in myosin V processivity experiments. It has also been used to provide biotin tags for neutravidin coating and also to suppress nonspecific cell adhesion during preparation of tension gauge tether (TGT) surface.
Physical form
Contains sodium citrate buffer salts
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) bioconjugation: suitable | technique(s) bioconjugation: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| assay ≥80% protein basis (biuret) | assay ≥80% protein basis (biuret) | assay ≥90% protein basis (biuret) | assay ≥96% (agarose gel electrophoresis) |
| biological source bovine | biological source bovine | biological source bovine | biological source bovine |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Taikopaul Kaneko et al.
Science advances, 6(4), eaax7413-eaax7413 (2020-02-06)
Kinesin is a motor protein that plays important roles in a variety of cellular functions. In vivo, multiple kinesin molecules are bound to cargo and work as a team to produce larger forces or higher speeds than a single kinesin.
Tilting and Wobble of Myosin V by High-Speed Single-Molecule Polarized Fluorescence Microscopy
Beausang JF, et al.
Biophysical Journal, 104, 1263-1273 (2019)
Integrin Molecular Tension within Motile Focal Adhesions
Wang X, et al.
Biophysical Journal, 109, 2259-2267 (2019)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A8549-10MG | 04061833392201 |
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Does the product, cat. no. A8549, can be used for developing serological test like Lateral Flow Assay (LFA)?
1 answer-
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How many biotin moles are there per mole of BSA of Product A8549, Albumin, biotin labeled bovine?
1 answer-
As listed in the "Properties" section of the product detail page, this material will have 8-16 moles of biotin per mole of BSA. The exact result of biotin moles will be listed on the lot specific certificate of analysis.
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What is the molecular weight for biotin labeled bovine(Product A8549, Albumin)?
1 answer-
In order to determine the molecular weight of this product, review the lot specific Certificate of Analysis for the Degree of Substitution (this provides the moles of biotin per mol of albumin). For lot SLCM9516, the Degree of Substitution is 8 mols biotin per mol of albumin. 8 x 244.31 (MW of biotin) + 66,430 (MW of BSA) = 68,384
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What is the linker between the BSA and the biotin for Product A8549, Albumin, biotin labeled bovine?
1 answer-
There is no linker or "spacer". The link between the BSA and the biotin is an amide bond connecting an amino group on the BSA to the carboxy group on the biotin.
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Do you have the amino acid sequence for bovine serum albumin?
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Bovine Serum Albumin is produced as a protein of 607 amino acids. The signal peptide (amino acids 1-18) and the propeptide (amino acids 19-22) are cleaved to make the mature form. The mature form of BSA is 583 amino acids (amino acids 25-607, Uniprot P02769). The sequence for the mature protein is below:DTHKSEIAHRFKDLGEEHFKGLVLIAFSQYLQQCPFDEHVKLVNELTEFAKTCVADESHAGCEKSLHTLFGDELCKVASLRETYGDMADCCEKQEPERNECFLSHKDDSPDLPKLKPDPNTLCDEFKADEKKFWGKYLYEIARRHPYFYAPELLYYANKYNGVFQECCQAEDKGACLLPKIETMREKVLTSSARQRLRCASIQKFGERALKAWSVARLSQKFPKAEFVEVTKLVTDLTKVHKECCHGDLLECADDRADLAKYICDNQDTISSKLKECCDKPLLEKSHCIAEVEKDAIPENLPPLTADFAEDKDVCKNYQEAKDAFLGSFLYEYSRRHPEYAVSVLLRLAKEYEATLEECCAKDDPHACYSTVFDKLKHLVDEPQNLIKQNCDQFEKLGEYGFQNALIVRYTRKVPQVSTPTLVEVSRSLGKVGTRCCTKPESERMPCTEDYLSLILNRLCVLHEKTPVSEKVTKCCTESLVNRRPCFSALTPDETYVPKAFDEKLFTFHADICTLPDTEKQIKKQTALVELLKHKPKATEEQLKTVMENFVAFVDKCCAADDKEACFAVEGPKLVVSTQTALA
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What is the method of preparation for Product A8549, Albumin, biotin labeled bovine?
1 answer-
The method of preparation of this product is considered proprietary by Sigma-Aldrich.
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What is Product A8549, Albumin, biotin labeled bovine, soluble in?
1 answer-
This product is soluble in water. This product contains sodium citrate buffer salts, so addition of a buffer is probably not necessary; just adding water is sufficient.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the solution stability of Product A8549, Albumin, biotin labeled bovine?
1 answer-
Sigma Aldrich has not tested the stability of this product in solution. It is recommended to prepare fresh solutions.
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