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Anti-Horse IgG (whole molecule)−Peroxidase antibody produced in rabbit

Name: Anti-Horse IgG (whole molecule)−Peroxidase antibody produced in rabbit
Brand: SIGMA
Price: 462 USD
Availability: InStock

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Rabbit Anti-Horse IgG (whole molecule)−HRP

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Size/SKU	Availability	Price
2 mL	Check Cart for Availability	$462.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162385

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

DB, ELISA (d), IHC (p)

Citations:

$462.00

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Properties

biological source

rabbit

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:15,000, dot blot: 1:100,000-1:200,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Horse IgGs have seven subclasses ranging from IgG1 to IgG7. Equine IgG antibodies mainly regulate mucosal and systemic immunological responses and thereby, provide protection against disease-causing pathogens such as Streptococcus equi, and the horse flu virus. Horse IgG may also function to control the advancement of EHV-1 infection . Anti-horse IgG (whole molecule)−peroxidase antibody is specific for IgG in horses.

Immunogen

Horse IgG

Application

Anti-horse IgG (whole molecule)−peroxidase antibody may be used in dot blot (1:8,000-1:10,000), immunohistochemistry (formalin-fixed, paraffin-embedded sections, at 1:150 dilution) and direct ELISA (1:15,000).

The level of S2-specific antibodies in test samples of horse serum was determined by ELISA using HRP-conjugated rabbit anti-horse IgG at a 1:35000 dilution with incubation for 45 minutes at room temperature.

Biochem/physiol Actions

IgG, a monoclonal antibody can be cleaved at the hinge region by nonspecific proteases like papain and pepsin. This can result in univalent Fab (antigen-binding fragments) fragments or bivalent F(ab′)2 fragments. These two enzymes have a broad substrate specificity resulting in heterogenous fragments.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A8667	A2054	A5670
$Anti-Horse IgG (whole molecule)−Peroxidase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich A9292 Anti-Horse IgG (whole molecule)−Peroxidase antibody produced in rabbit	$Anti-Human IgG (whole molecule)−Peroxidase antibody produced in goat IgG fraction of antiserum, buffered aqueous solution$ Sigma-Aldrich A8667 Anti-Human IgG (whole molecule)−Peroxidase antibody produced in goat	Sigma-Aldrich A2054 Anti-Monkey IgG (whole molecule)−Peroxidase antibody produced in rabbit	Sigma-Aldrich A5670 Anti-Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit
description IgG fraction of antiserum, buffered aqueous solution	description IgG fraction of antiserum, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution
biological source rabbit	biological source goat	biological source rabbit	biological source rabbit
technique(s) direct ELISA: 1:15,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150, dot blot: 1:100,000-1:200,000 (chemiluminescent)	technique(s) direct ELISA: 1:30,000, dot blot: 1:80,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200	technique(s) direct ELISA: 1:45,000, dot blot: 1:160,000 (direct chemiluminescence assay)	technique(s) direct ELISA: 1:40,000, dot blot: 1:150,000 (chemiluminescent)
antibody form IgG fraction of antiserum	antibody form IgG fraction of antiserum	antibody form affinity isolated antibody	antibody form affinity isolated antibody
conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice

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pictograms

GHS08

signalword

Danger

hcodes

H317,H334

pcodes

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c