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A9292

Anti-Horse IgG (whole molecule)−Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Rabbit Anti-Horse IgG (whole molecule)−HRP

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2 mL
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$462.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162385
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
DB, ELISA (d), IHC (p)
Citations:
8

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biological source

rabbit

Quality Segment

conjugate

peroxidase conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:15,000, dot blot: 1:100,000-1:200,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Horse IgGs have seven subclasses ranging from IgG1 to IgG7. Equine IgG antibodies mainly regulate mucosal and systemic immunological responses and thereby, provide protection against disease-causing pathogens such as Streptococcus equi, and the horse flu virus. Horse IgG may also function to control the advancement of EHV-1 infection . Anti-horse IgG (whole molecule)−peroxidase antibody is specific for IgG in horses.

Immunogen

Horse IgG

Application

Anti-horse IgG (whole molecule)−peroxidase antibody may be used in dot blot (1:8,000-1:10,000), immunohistochemistry (formalin-fixed, paraffin-embedded sections, at 1:150 dilution) and direct ELISA (1:15,000).
The level of S2-specific antibodies in test samples of horse serum was determined by ELISA using HRP-conjugated rabbit anti-horse IgG at a 1:35000 dilution with incubation for 45 minutes at room temperature.

Biochem/physiol Actions

IgG, a monoclonal antibody can be cleaved at the hinge region by nonspecific proteases like papain and pepsin. This can result in univalent Fab (antigen-binding fragments) fragments or bivalent F(ab′)2 fragments.  These two enzymes have a broad substrate specificity resulting in heterogenous fragments.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Horse IgG (whole molecule)−Peroxidase antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

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Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in rabbit

description

IgG fraction of antiserum, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous glycerol solution

description

IgG fraction of antiserum, buffered aqueous solution

description

IgG fraction of antiserum, buffered aqueous solution

biological source

rabbit

biological source

rabbit

biological source

goat

biological source

rabbit

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

technique(s)

dot blot: 1:100,000-1:200,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150, direct ELISA: 1:15,000

technique(s)

direct ELISA: 1:30,000, western blot: 1:30,000

technique(s)

dot blot: 1:100,000 (chemiluminescent), direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

technique(s)

western blot (chemiluminescent): 1:5,000 using detecting rat primary antibody, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500, direct ELISA: 1:40,000

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

buffered aqueous solution

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

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Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Codes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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