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B0529

Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat anti-Mouse IgG

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0.5 mL

Available to ship TODAYfromMILWAUKEE

$236.00
1 mL

Available to ship TODAYfromMILWAUKEE

$344.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162621
Conjugate:
biotin conjugate
Clone:
polyclonal
Application:
ELISA (d), IHC (p), WB
Citations:
41

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biological source

goat

Quality Segment

conjugate

biotin conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

technique(s)

direct ELISA: 1:200,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300, western blot: 1:300,000-1:600,000 using using Hela cells extract

storage temp.

−20°C

target post-translational modification

unmodified

General description

Binds all mouse Igs.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Each immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

Application

Anti-Mouse IgG (Fab specific)-Biotin antibody produced in goat has been used in immunohistochemistry and immunofluorescence studies.
Anti-Mouse IgG (Fab specific)-Biotin antibody produced in goat is suitable for direct ELISA at a dilution of 1:200000 and for immunohistochemistry at a dilution of 1:300. The antibody was used to stain paraffin-embedded, rat skin, duodenum, esophagus and spleen sections at a dilution of 1:200. For immunofluorescence of Human Gingival Fibroblasts, a dilution of 1:200 was used. For immunoblotting experiments using mouse adipose tissue, antibody dilution of 1:30000 was used.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background staining with human or rat samples.

Other Notes

Antibody adsorbed with human IgG and rat serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat affinity isolated antibody, buffered aqueous solution

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description

affinity isolated antibody, buffered aqueous solution

description

2.0 mg/mL, affinity isolated antibody

description

affinity isolated antibody, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous solution

biological source

goat

biological source

goat

biological source

goat

biological source

goat

conjugate

biotin conjugate

conjugate

unconjugated

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

technique(s)

western blot: 1:300,000-1:600,000 using using Hela cells extract, direct ELISA: 1:200,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300

technique(s)

indirect ELISA: 1:15,000, Ouchterlony double diffusion: suitable

technique(s)

direct ELISA: 1:60,000, western blot: 1:80,000-1:160,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

technique(s)

direct ELISA: 1:50,000, western blot: 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue section

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

species reactivity

mouse

species reactivity

-

species reactivity

mouse

species reactivity

mouse

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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Storage Class

10 - Combustible liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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