The B1267 Biotin-maleimide is a white powder that is soluble in DMSO at 10mg/ml, resulting in a clear colorless solution. A stock solution of the product in DMSO at 20 mg/ml can be prepared without the need for heat. This solution can then be diluted 1:10 with an aqueous buffer system, such as PBS or other. Concentrations of 40-50 mg/ml can also be prepared, but heat, i.e. 40°C, is needed. For most applications, a stock of 20 mg/ml would be suitable.
B1267
Biotin-maleimide
≥95% (TLC), powder
Synonym(s):
N-Biotinoyl-N′-(6-maleimidohexanoyl)hydrazide
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| Size/SKU | Availability | Price |
|---|---|---|
25 mg | Available to ship TODAYfromMILWAUKEE | $166.00 |
100 mg | Available to ship TODAYfromMILWAUKEE | $526.00 |
About This Item
Empirical Formula (Hill Notation):
C20H29N5O5S
CAS Number:
Molecular Weight:
451.54
MDL number:
UNSPSC Code:
12352203
PubChem Substance ID:
NACRES:
NA.46
Quality Segment
assay
≥95% (TLC)
form
powder
solubility
acetic acid: 20 mg/mL
storage temp.
2-8°C
SMILES string
O=C(CCCCCN1C(=O)C=CC1=O)NNC(=O)CCCC[C@@H]2SC[C@@H]3NC(=O)N[C@H]23
InChI
1S/C20H29N5O5S/c26-15(7-2-1-5-11-25-17(28)9-10-18(25)29)23-24-16(27)8-4-3-6-14-19-13(12-31-14)21-20(30)22-19/h9-10,13-14,19H,1-8,11-12H2,(H,23,26)(H,24,27)(H2,21,22,30)/t13-,14-,19-/m0/s1
InChI key
VAPZWEFPQRDVHR-NJSLBKSFSA-N
General description
Biotin-maleimide is a biotin specific thiol containing reagent used to biotinylate different proteins through artificially induced or natural sulphadryl group. It can be used as a universal, multipurpose, thiol-specific probe. Biotin-maleimide can also be used in dot blots to detect protein SH groups.
Application
Biotin-maleimide- 0.01 mg/ml in PBS containing 0.1% Tween 20, incubated for 1 h at room temperature can be used to detect deprotected cysteine thiols present on immobilized peptides.
Sulfhydryl-specific biotinylation reagent. Couples by highly efficient thioether linkage. Typically used to couple to intrinsic sulfhydryls, e.g. protein, and avoid modification of primary amine.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| description ≥95% (TLC), powder | description ≥97% (TLC), powder | description ≥95% | description - |
| assay ≥95% (TLC) | assay ≥97% (TLC) | assay ≥95% | assay - |
| form powder | form powder | form powder | form lyophilized powder |
| solubility acetic acid: 20 mg/mL | solubility DMSO: ≤20 mg/mL | solubility - | solubility - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What is the solubility of B1267 Biotin-maleimide in DMSO?
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