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Anti-Goat/Sheep IgG−Biotin antibody, Mouse monoclonal

Name: Anti-Goat/Sheep IgG−Biotin antibody, Mouse monoclonal
Brand: SIGMA
Price: 186 USD
Availability: InStock

clone GT-34, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Goat/Sheep IgG, Monoclonal Anti-Goat/Sheep IgG–Biotin antibody produced in mouse

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Size/SKU	Availability	Price
0.2 mL	Available to ship TODAYfromMILWAUKEE	$186.00

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

biotin conjugate

Clone:

GT-34, monoclonal

Application:

ELISA (d)

Citations:

$186.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

biotin conjugate

antibody form

purified from hybridoma cell culture

antibody product type

secondary antibodies

clone

GT-34, monoclonal

form

buffered aqueous solution

species reactivity

sheep, goat, bovine

should not react with

rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human

technique(s)

direct ELISA: 1:5,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Monoclonal Anti-Goat IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2.

The product binds to all goat/sheep IgG1

Application

Monoclonal Anti-Goat/Sheep IgG-Biotin antibody produced in mouse has been used in:

enzyme linked immuno sorbent assay (ELISA)
immunoblotting
dot blotting
immunohistochemistry
immunocytochemistry

Monoclonal Anti-Goat/Sheep IgG-Biotin antibody produced in mouse may be used for ELISA at a minimum working dilution of 1:5000. It was used at a working dilution of 1:1000 to visualize collagen type IV in mouse digit sections.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin allows for the binding of various labels such as avidin or streptavidin.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	G2904	B6775	B3398
Sigma-Aldrich B3148 Anti-Goat/Sheep IgG−Biotin antibody, Mouse monoclonal	Sigma-Aldrich G2904 Monoclonal Anti-Goat/Sheep IgG antibody produced in mouse	Sigma-Aldrich B6775 Anti-Human IgG1−Biotin antibody, Mouse monoclonal	Sigma-Aldrich B3398 Anti-Human IgG2−Biotin antibody, Mouse monoclonal
description clone GT-34, purified from hybridoma cell culture	description clone GT-34, ascites fluid	description clone 8c/6-39, purified from hybridoma cell culture	description clone HP-6014, purified from hybridoma cell culture
antibody form purified from hybridoma cell culture	antibody form ascites fluid	antibody form purified from hybridoma cell culture	antibody form purified from hybridoma cell culture
species reactivity sheep, goat, bovine	species reactivity sheep, goat, bovine	species reactivity -	species reactivity human
biological source mouse	biological source mouse	biological source mouse	biological source mouse
technique(s) direct ELISA: 1:5,000	technique(s) indirect ELISA: 1:10,000-1:20,000	technique(s) direct ELISA: 1:1,000	technique(s) direct ELISA: 1:30,000
conjugate biotin conjugate	conjugate unconjugated	conjugate biotin conjugate	conjugate biotin conjugate
isotype IgG1	isotype IgG1	isotype IgG2a	isotype IgG1

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Storage Class

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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