B3679
BCIP®/NBT-Blue
alkaline phosphatase substrate, chromogenic, liquid
Synonym(s):
5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride liquid substrate system
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 mL | Please contact Customer Service for Availability | $313.00 |
About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
$313.00
Please contact Customer Service for Availability
Product Name
BCIP®/NBT-Purple Liquid Substrate System for Membranes, ready to use alkaline phosphatase substrate
Quality Level
form
liquid
storage temp.
2-8°C
Application
BCIP®/NBT-Purple Liquid Substrate System for Membranes has been used as a substrate for alkaline phosphatase
- for the detection of secondary antibodies by immunoblot
- in ELISPOT assay
- in dot blot analysis
- in western blot to visualize protein bands on nitrocellulose membrane
Biochem/physiol Actions
5-bromo-4-chloro-3-indolyl phosphate (BCIP)/ nitro blue tetrazolium (NBT) is a chromogenic substrate system for the detection of alkaline phosphatase (AP) enzyme. AP hydrolyses BCIP to form an intermediate leucoindigo compound and is further oxidized by NBT to form 5,5′-dibromo-4,4′-dichloro indigo (BCI). NBT is reduced to an insoluble purple diformazan (DF) and forms NBT-DF. A mixture of NBT-DF and BCI results in the dark purple colored precipitate. AP by hydrolysing many BCIP molecules makes it a highly sensitive method.
Physical form
Ready-to-use.
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Stable at least one year at 2-8 °C.
1 of 1
This Item | |||
|---|---|---|---|
| Quality Level 100 | Quality Level 300 | Quality Level 100 | Quality Level - |
| form liquid | form liquid | form liquid | form solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
Still not finding the right product?
Explore all of our products under BCIP®/NBT-Purple Liquid Substrate System for Membranes
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
Related Content
Characterization of the 41 kDa allergen Asp v 13, a subtilisin-like serine protease from Aspergillus versicolor
Shi C and Miller JD
Molecular Immunology, 48(15-16), 1827-1834 (2011)
Membrane bioreactor separator system for integrated IgG fragmentation and Fab purification
Yu D and Ghosh R
Journal of Immunological Methods, 359(1-2), 37-41 (2010)
Xudong Wang et al.
Frontiers in cell and developmental biology, 9, 690502-690502 (2021-08-28)
Melatonin (Mlt) confers potential antitumor effects in various types of cancer. However, to the best of our knowledge, the role of Mlt in the giant cell tumor of bone (GCTB) remains unknown. Moreover, further research is required to assess whether
Global Trade Item Number
| SKU | GTIN |
|---|---|
| B3679-100ML | 04061833428351 |